We narrowed to 4,771 results for: IRES

1. SHP2(T253M/Q257L)@pLKO-Trex-SBP

   Plasmid

   #85458

   Purpose

   SHP2 mutant gene expression

   Depositor

   Darrin Stuart

   Article

   Chen et al Nature. 2016 Jul 7;535(7610):148-52.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Lentiviral

   Tags

   SBP Tag

   Expression

   Mammalian

   Mutation

   T253M/Q257L

   Promoter

   RSV

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. EH1

   Bacterial Strain

   #102804

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. RF17

   Bacterial Strain

   #102801

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

   Depositor

   Robert Gennis, Toshio Iwasaki

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pKP332 (Lenti-OSK1)

   Plasmid

   #21627

   Depositor

   Tim Townes

   Article

   Chang et al Stem Cells. 2009 May . 27(5):1042-9.

   Insert

   hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIH-mRuby2-BAX

   Plasmid

   #111628

   Purpose

   Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   Bax (Bax Mouse)
   

   Use

   Retroviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TET-MKOS

   Plasmid

   #20959

   Purpose

   piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIH-mRuby2-BAK

   Plasmid

   #111627

   Purpose

   Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   BAK (Bak1 Mouse)
   

   Use

   Retroviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PIG-Myc

   Plasmid

   #177650

   Purpose

   Expression of wild-type mouse Myc

   Depositor

   Trudy Oliver

   Article

   Mollaoglu et al Cancer Cell. 2017 Feb 13;31(2):270-285.

   Insert

   Myc (Myc Mouse)
   

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_GRB10_WT

    Plasmid

    #82124

    Purpose

    Gateway Donor vector containing GRB10 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    GRB10 (GRB10 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-FFSS-Cyclin D1

    Plasmid

    #215151

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215152

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LeGO-iG-CSF2RB(A455D)

    Plasmid

    #125751

    Purpose

    Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

    Depositor

    Dirk Heckl

    Article

    Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

    Insert

    CSF2RB(A455D)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A455D, codon optimized

    Promoter

    SFFV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI AscI MR1 R9H res

    Plasmid

    #214753

    Purpose

    expresses human MR1 R9H resistant to CRISPR editing with a specific sgRNA in mammalian cells

    Depositor

    David Lewinsohn

    Article

    Kulicke et al Commun Biol. 2024 Feb 24;7(1):228. doi:

    Insert

    MHC class I-related protein 1 (MR1 Human)
    

    Use

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    silent mutations to prevent editing by CRISPR/Cas…

    Promoter

    CMV

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-V1-rCD20-BSD

    Plasmid

    #209752

    Purpose

    To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-V2-rCD20-BSD

    Plasmid

    #209753

    Purpose

    To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-V3-rCD20-BSD

    Plasmid

    #209754

    Purpose

    To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid

    #74055

    Purpose

    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
    

    Use

    Retroviral

    Tags

    AVI-TEV and FLAG

    Expression

    Mammalian

    Mutation

    silent mutation A1723C in the sequence of NM_1730…

    Promoter

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RF15

    Bacterial Strain

    #102799

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

    Depositor

    Robert Gennis, Toshio Iwasaki

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-FLuc

    Plasmid

    #170575

    Purpose

    Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    Luc
    

    Use

    Luciferase and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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