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pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709)

Plasmid

#80004

Purpose

miRFP709 GAF domain fused to FKBP and NLS

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Tags

GAF domain of miRFP709 and NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10A-V5

Plasmid

#35939

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-T1ER

Plasmid

#47928

Purpose

T1ER is a calcium sensor targeted to ER

Depositor

Tobias Meyer

Article

Bandara et al Sci Signal. 2013 Jul 9;6(283):ra56. doi:

Insert

T1ER

Expression

Mammalian

Mutation

KDEL

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
Wnt1 STOP

Plasmid

#35868

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pJK_proB_eGFP

Plasmid

#59845

Purpose

Constitutively expresses eGFP with proB promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proB

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

G324D

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

dTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJL-mGold

Plasmid

#157997

Purpose

Expression of mGold in yeast cells

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

mGold

Expression

Yeast

Mutation

mGold is mVenus with L46F;T63S mutations

Promoter

pTDH(GAP promoter)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60626

Purpose

Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP16 mouse MyoD

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_eGFP

Plasmid

#59853

Purpose

Constitutively expresses eGFP with proK1 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK1

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pDRF1 mScarlet1.4-T2A-mTurquoise2

Plasmid

#219849

Purpose

equimolar expression of mScarlet1.4 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mScarlet1.4-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 mStayGold-T2A-mCherry

Plasmid

#219834

Purpose

equimolar expression of mStaygold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10B-V5

Plasmid

#35940

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSG81-pLas-tetO-deGFPssrA

Plasmid

#102454

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Tags

ssrA

Expression

Bacterial

Promoter

pLas-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Promoter

PccmK2

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDRF1 ymStayGold-T2A-mCherry

Plasmid

#219843

Purpose

equimolar expression of ymStayGold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSP72-deltaFlincG

Plasmid

#19733

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

delta-FlincG (PRKG1 Bovine)

Tags

His

Expression

Bacterial

Mutation

aa 77-356

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only