222,697 results

1. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60228

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-SLC25A1_STOP

   Plasmid

   #161465

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A1 (SLC25A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCdOpt-BMX

   Plasmid

   #203929

   Purpose

   This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

   Depositor

   Scott Briggs

   Article

   Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

   Insert

   BleMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   BleMX was codon optimized for Candida albicans or…

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET20b- hTPI

   Plasmid

   #50723

   Purpose

   overexpression of human TPI1 in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET20b- hTPI

   Plasmid

   #50723

   Purpose

   overexpression of human TPI1 in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMAL-CellTag-V3

   Pooled Library

   #115645

   Purpose

   Viral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamics

   Depositor

   Samantha Morris

   Article

   Biddy et al Nature. 2018 Dec;564(7735):219-224. doi:

   Has Service

   Concentrated Lentiviral Prep

   Expression

   Mammalian

   Species

   Synthetic

   Use

   Lentiviral

   Available since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

9. mEmerald-Desmin-C-18

   Plasmid

   #54059

   Purpose

   Localization: Intermediate Filaments, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Desmin (DES Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. mEmerald-Desmin-C-18

    Plasmid

    #54059

    Purpose

    Localization: Intermediate Filaments, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Desmin (DES Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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