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pEYFP-Syx1

Plasmid

#35709

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
mg171

Plasmid

#34651

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B0;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg173

Plasmid

#34653

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B2;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg174

Plasmid

#34654

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg175

Plasmid

#34655

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg176

Plasmid

#34656

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B2;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg177

Plasmid

#34657

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg178

Plasmid

#34658

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg179

Plasmid

#34659

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B2;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
mg181

Plasmid

#34661

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B1;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pOX + JShal XR

Plasmid

#16148

Depositor

Larry Salkoff

Article

Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

Insert

Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pJC45BS104 Y257W

Plasmid

#1303

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 257 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pJC45BS104 Y507W

Plasmid

#1305

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 507 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b F130W

Plasmid

#1309

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Phe 130 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b Y257W

Plasmid

#1310

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Tyr 257 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b Y466W

Plasmid

#1311

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y466W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Tyr 466 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b Y507W

Plasmid

#1312

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Tyr 507 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b L553W

Plasmid

#1313

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Leu 553 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b Y819W

Plasmid

#1314

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y819W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Tyr 819 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104b L892W

Plasmid

#1315

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Expression

Yeast

Mutation

Changed Leu 892 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbE8k-fcs

Plasmid

#83068

Purpose

Expresses Fcs under pBAD regulation

Depositor

Matthew Chang

Article

Lo et al Cell Syst. 2016 Aug;3(2):133-43. doi: 10

Insert

fcs

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

Enlarge
enz.043-SETD7-Y305F

Plasmid

#154045

Purpose

Expresses SETD7 (Y305F) in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone-lysine N-methyltransferase SETD7 (SETD7 Human)

Tags

His, MBP

Expression

Bacterial

Mutation

changed tyrosine 305 to phenylalanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) F234C

Plasmid

#170644

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

C277A, F234C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) V304C

Plasmid

#170645

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) D303G F234C

Plasmid

#170647

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G, C277A, F234C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) D303G V304C

Plasmid

#170648

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D303G, C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) D365E F234C

Plasmid

#170650

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E, C277A, F234C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) D365E V304C

Plasmid

#170651

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

D365E, C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) I367T F234C

Plasmid

#170653

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T, C277A, F234C

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Syt1 C2AB (96-421) I367T V304C

Plasmid

#170654

Purpose

Expression of syt1 for purification in E. Coli

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Expression

Bacterial

Mutation

I367T, C277A, V304C

Availability

Academic Institutions and Nonprofits only

Enlarge
Human CRISPR Metabolic Gene Knockout Library

Pooled Library

#110066

Purpose

Targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene.

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Use

Lentiviral

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only
p11-5’_10xN_PAM-site3 (RTW572)

Pooled Library

#160134

Purpose

Plasmid library harboring a randomized 10nt PAM 5’ of target site 3. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines

Pooled Library

#86538

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
attB GPCR Library with Unique Molecular Identifiers

Pooled Library

#243681

Purpose

For stable recombination of nearly all human GPCRs with unique molecular identifiers.

Depositor

Jonathan Schlebach

Article

Tedman et al bioRxiv 2025.09.19.677468

Expression

Mammalian

Species

Homo sapiens

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only
attB Barcoded Canonical GPCR Library

Pooled Library

#243682

Purpose

For stable recombination of nearly all human GPCRs with unique molecular identifiers.

Depositor

Jonathan Schlebach

Article

Tedman et al bioRxiv 2025.09.19.677468

Expression

Mammalian

Species

Homo sapiens

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only
p11-3’_8xN_PAM-site1 (RTW554)

Pooled Library

#160132

Purpose

Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - Unique to HEK293T

Pooled Library

#86543

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
p11-5’_10xN_PAM-site4 (RTW574)

Pooled Library

#160135

Purpose

Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Species

Synthetic

Use

CRISPR

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only
CRISPRi non-coding library (CRiNCL) - iPSC & HFF

Pooled Library

#86550

Depositor

Jonathan Weissman

Article

Liu et al Science. 2017 Jan 06. Vol. 355, Issue 63

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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