We narrowed to 13,654 results for: crispr cas9

1. pSaGuide

   Plasmid

   #64710

   Purpose

   Cloning and expression of Staphylococcus aureus guide RNA

   Depositor

   Kiran Musunuru

   Insert

   Staphylococcus aureus guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMK

   Plasmid

   #62818

   Purpose

   Bacterial constitutive S. pyogenes Cas9 expression plasmid

   Depositor

   Sébastien Rodrigue

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. DS-SPcas

   Plasmid

   #48645

   Purpose

   Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   tracrRNA precursor
   

   Use

   CRISPR

   Promoter

   proC and tracrRNA promoter

   Available Since

   Dec. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. M-ST1cas

   Plasmid

   #48669

   Purpose

   Mammalian S. thermophilus #1 Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Promoter

   CMV

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. M-NMcas

   Plasmid

   #48670

   Purpose

   Mammalian N. meningitidis Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Promoter

   CMV

   Available Since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

6. DS-NMcas

   Plasmid

   #48646

   Purpose

   Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   NM tracrRNA
   

   Use

   CRISPR

   Promoter

   NM tracrRNA promoter and proC

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. M-SPcas

   Plasmid

   #48668

   Purpose

   Mammalian S. pyogenes Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Promoter

   CMV

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. DS-ST1cas

   Plasmid

   #48647

   Purpose

   Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   ST1 tracrRNA
   

   Use

   CRISPR

   Promoter

   proC

   Available Since

   Nov. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. BCJJ344

   Plasmid

   #117503

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtUBI10

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS544

    Plasmid

    #188882

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-dSa-VPR

    Plasmid

    #99651

    Purpose

    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

    Depositor

    George Church

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65-RTA activator

    Mutation

    dead Cas9

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV/Guide-mCherry

    Plasmid

    #170543

    Purpose

    Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Promoter

    Ef1-alpha

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.5’eGFP/sgLMNA

    Plasmid

    #170546

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of LMNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGE011

    Plasmid

    #71537

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGE_En03

    Plasmid

    #71535

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Sumihare Noji, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGE010

    Plasmid

    #71536

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge