We narrowed to 21,361 results for: NTS;

1. pcDNA3-MAVS-M142A(KaganF59)

   Plasmid

   #52062

   Purpose

   Expresses the human MAVS CDS containing the point mutation M142A "weak translational context"

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M142A
   

   Expression

   Mammalian

   Mutation

   changed Met 142 to Ala

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

   Plasmid

   #50899

   Purpose

   Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A720C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

   Plasmid

   #50897

   Purpose

   Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   L718C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

   Plasmid

   #50901

   Purpose

   Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   L722C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)

   Plasmid

   #50895

   Purpose

   Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   S716C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)

   Plasmid

   #50900

   Purpose

   Expresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   I721C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)

   Plasmid

   #50908

   Purpose

   Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   C723S C724V W732C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)

   Plasmid

   #50903

   Purpose

   Expresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   C722S C723V I724C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)

   Plasmid

   #50904

   Purpose

   Expresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   C723S C724V V726C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)

    Plasmid

    #50898

    Purpose

    Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    G719C C723S C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

    Plasmid

    #52007

    Purpose

    Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    5utr-MAVS-ORF2,3mut
    

    Expression

    Mammalian

    Mutation

    mutated start codons for ORF3 and 4

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pJK637

    Plasmid

    #72437

    Purpose

    Acetobacter aceti 1023 mutS, partial gene

    Depositor

    T. J. Kappock

    Insert

    DNA mismatch repair protein MutS (partial gene)
    

    Expression

    Bacterial

    Mutation

    deletes nt 1-767 and nt 2086-2655 of mutS gene

    Promoter

    n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSELIS-RamR

    Plasmid

    #202631

    Purpose

    Enables dual growth and fluorescence based enrichment of RamR biosensors

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    Sh ble
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Lambda promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pBW842_pCAG-pExpr-FP-DECODER

    Plasmid

    #87551

    Purpose

    Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP, EGFP, iRFP720, mRuby2
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRL1

    Plasmid

    #153997

    Purpose

    sgRNA in vitro transcription plasmid.

    Depositor

    Shengdar Tsai

    Article

    Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

    Type

    Empty backbone

    Use

    CRISPR; Sgrna under a t7 promoter for in vitro tr…

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-LRR

    Plasmid

    #66937

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only