Addgene

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pEZYpci

Plasmid

#18707

Purpose

pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pMN cFKT

Plasmid

#18785

Depositor

Han Htun

Article

Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

Insert

TRAP (mtrB Bacillus subtilis)

Use

Tags

6xHis and HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.mCherry-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187102

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Use

AAV

Tags

mCherry-P2A

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187101

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Use

AAV

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Left-stuffer-8e-E1347A

Plasmid

#187163

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-8e-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero7

Plasmid

#187926

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero7

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero7 (SERF2 Human)

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero9

Plasmid

#187927

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero9

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero9

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero13

Plasmid

#187929

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero13

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero13 (BEX3 Human)

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero20

Plasmid

#187930

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero20

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero20 (C11orf58 Human)

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero45

Plasmid

#187931

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero45

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero45 (SERBP1 Human)

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Article

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

Plasmid

#187994

Purpose

Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

mucin2 D1D2D'D3 (MUC2 Human)

Use

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mTurbo-G

Plasmid

#187176

Purpose

Express miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Article

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-EGFP-WPRE

Plasmid

#187103

Purpose

Double floxed EGFP under the control of EF1a promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagBcl10K31RK63R

Plasmid

#18719

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Lysine 31 to arginine and Lysine 63 to arginine.

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
Cdk4-DN

Plasmid

#1875

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative, D to N in conserved KLAD*FGLAR…

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pBAD_dGFPmut3-AAV

Plasmid

#187379

Purpose

arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Insert

dGFPmut3_AAV

Use

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_ROR2

Plasmid

#187759

Purpose

MAC-tagged gene expression of human ROR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROR2 (ROR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_NTRK3

Plasmid

#187754

Purpose

MAC-tagged gene expression of human NTRK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

NTRK3 (NTRK3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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