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Addgene

We narrowed to 15,195 results for: AMPH

Showing: 421 - 440 of 15195 results
  1. pDEINO4

    Plasmid
    #179488
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Insert
    aadA1
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pDEINO3

    Plasmid
    #179487
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Insert
    TetR/A
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO, native, native (respectively)
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pSV014

    Plasmid
    #214740
    Purpose
    High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation
    Depositor
    Insert
    BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pXC3cIfm

    Plasmid
    #193750
    Purpose
    Expresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selection
    Depositor
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLUX
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pLT237

    Plasmid
    #174300
    Purpose
    Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.
    Depositor
    Insert
    PolC homology arm
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Cysteine 669 to Tyrosine
    Promoter
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pCm N-term SMT3 NikJC199U

    Plasmid
    #174210
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pCm C-term SMT3 NikJC199U

    Plasmid
    #174362
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pCm N-term MBP NikJC199U

    Plasmid
    #174364
    Purpose
    Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. ARK1a

    Plasmid
    #81016
    Purpose
    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)
    Depositor
    Inserts
    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    Use
    Tags
    Expression
    Mutation
    Promoter
    LacUV5 and Trc promoter
    Available since
    Aug. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pCm C-term MBP NikJC199U

    Plasmid
    #174365
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 421 - 440 of 15195 results