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Plasmid#36859DepositorInsertSyndapin1 (Pacsin1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
C77m
Plasmid#120989PurposeMoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAmpR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
NET29A.Hs pRRLSIN (1742)
Plasmid#62052Purposelentiviral expression of nuclear envelope transmembrane proteinDepositorInsertNET29 (TMEM120A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTargetAmp
Plasmid#228552PurposeTemplate for PCR amplification for gRNA cloning plasmid by in vivo cloning.DepositorInsertAmpicillin resistance
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-Reps1-EH domain
Plasmid#36860DepositorInsertReps1 (REPS1 Human)
UseTagsGSTExpressionBacterialMutationdeleted amino acids 1-225 and 315-769PromoterAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS2ndd
Plasmid#97353PurposeLow background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, ampicillin resistantDepositorInsertNucleoid Disruptive Domain
UseCloningTagsExpressionBacterialMutationchanged methionine 1 to isoleucinePromoterE. coli lacZ promoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHflu2
Plasmid#203880PurposepHI1 with insertion of ampicillin-resistance geneDepositorInsertbroad-spectrum class A beta-lactamase TEM-116
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gutB
Plasmid#212139PurposeCargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertxylitol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1_EHD1_438-534
Plasmid#36459DepositorInsertEHD1 EH domain (EHD1 Human)
UseTagsGSTExpressionBacterialMutationdeleted amino acids 1-437 from full-length constr…PromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMulti_A_ccdB
Plasmid#223851PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Nix
Plasmid#100759PurposeBacterial expression of GST fused Nix proteinDepositorInsertGST-Nix (BNIP3L Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAM18dRGA[MCS]
Plasmid#84683PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Nix-S212D
Plasmid#100761PurposeBacterial expression of GST fused Nix-S212D proteinDepositorInsertGST-Nix-S212D (BNIP3L Human)
UseTagsGSTExpressionBacterialMutationS212DPromotertacAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Nix-S212A
Plasmid#100760PurposeBacterial expression of GST fused Nix-S212A proteinDepositorInsertGST-Nix-S212A (BNIP3L Human)
UseTagsGSTExpressionBacterialMutationS212APromotertacAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain A (IRESneo)
Plasmid#82450PurposeChain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.DepositorInsertrFIXX-bp chain A
UseTagsPolyhistidineExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
rFIXX-bp chain B (IRESpuro)
Plasmid#82451PurposeChain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.DepositorInsertrFIXX-bp chain B
UseTagsBiotinExpressionBacterial and MammalianMutationPromoterT7 RNA polymerase promoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_AH
Plasmid#217602PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AH
Plasmid#217606PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_AF
Plasmid#217604PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only