We narrowed to 1,086 results for: gibson assembly

1. pFP.R271

   Plasmid

   #138220

   Purpose

   Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOPO Venus

   Plasmid

   #68493

   Purpose

   TOPO construct expressing Venus for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   split Venus
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EZ-L498

   Plasmid

   #131771

   Purpose

   PPGK1_FUSN_Citrine _PixE_TCYC1

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   PPGK1_FUSN_Citrine _PixE_TCYC1
   

   Use

   Integration into his3 locus

   Expression

   Yeast

   Promoter

   PGK

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L SpyTag-ELP-SpyTag DK

   Plasmid

   #112635

   Purpose

   SpyTag linked by an elastin-like polypeptide to non-reactive SpyTag DK

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   SpyTag-ELP-SpyTag DK
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Contains central cysteine. Reactive aspartic acid…

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFP.R365

   Plasmid

   #138235

   Purpose

   Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO CloverCP

   Plasmid

   #68444

   Purpose

   TOPO construct expressing CloverCP for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   CloverCP
   

   Use

   Gmap

   Tags

   "CP" degradation domain

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOPO mT2

   Plasmid

   #68491

   Purpose

   TOPO construct expressing mT2 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mTurquoise2
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EBFP2-PIPKγ87

   Plasmid

   #242824

   Purpose

   Encodes wildtype human PIPKγ87 and N-terminal EBFP2 tag

   Depositor

   Cheng-han Yu

   Article

   Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

   Insert

   Phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
   

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSuper TR G414C

   Plasmid

   #207612

   Purpose

   U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   Telomerase RNA G414C
   

   Expression

   Mammalian

   Promoter

   U1

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26b(modified)-T-Lg

    Plasmid

    #216977

    Purpose

    Expression of GvpT-Linker-Large BiT

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    GvpT-Linker-Large BiT
    

    Tags

    Split Nanoluciferase LargeBiT-FLAG

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pST39-Mega'-deltaRLS

    Plasmid

    #216955

    Purpose

    Mega' with knockout of GvpR, GvpL and GvpS

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpR, GvpL and GvpS
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pST39-Mega'-deltaBSU

    Plasmid

    #216953

    Purpose

    Mega' with knockout of GvpB, GvpS and GvpU

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB, GvpS and GvpU
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST39-Mega'-deltaLSK

    Plasmid

    #216957

    Purpose

    Mega' with knockout of GvpL, GvpS and GvpK

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpL, GvpS and GvpK
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST39-Mega'-deltaBRG

    Plasmid

    #216945

    Purpose

    Mega' with knockout of GvpB, GvpR and GvpG

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB, GvpR and GvpG
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET26b(modified)-Sm-U

    Plasmid

    #217003

    Purpose

    Expression of Small BiT-Linker-GvpU

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Small BiT-Linker-GvpU
    

    Tags

    Split Nanoluciferase SmallBiT

    Expression

    Bacterial

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST39-Mega'-deltaBRS

    Plasmid

    #216947

    Purpose

    Mega' with knockout of GvpB, GvpR and GvpS

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB, GvpR and GvpS
    

    Expression

    Bacterial

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST39-Mega'-deltaBS

    Plasmid

    #216895

    Purpose

    Mega' with knockout of GvpB and GvpS

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB and GvpS
    

    Expression

    Bacterial

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST39-Mega'-deltaBLJ

    Plasmid

    #216951

    Purpose

    Mega' with knockout of GvpB, GvpL and GvpJ

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB, GvpL and GvpJ
    

    Expression

    Bacterial

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST39-Mega'-deltaBU

    Plasmid

    #216899

    Purpose

    Mega' with knockout of GvpB and GvpU

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB and GvpU
    

    Expression

    Bacterial

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST39-Mega'-deltaBJ

    Plasmid

    #216897

    Purpose

    Mega' with knockout of GvpB and GvpJ

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Mega' with knockout of GvpB and GvpJ
    

    Expression

    Bacterial

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge