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Showing: 421 - 440 of 10438 results

  2. bbCas9pluspAAA

    Plasmid
    #82581
    Purpose
    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
    Depositor
    Ivo Huijbers
    Article
    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    82581-plasmid-map-sequence-id-228465

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  4. env8 selectivity (d')

    Plasmid
    #99838
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (d')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99838-plasmid-map-sequence-id-192115

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  14. env8 7U J1/13

    Plasmid
    #99829
    Purpose
    GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:
    Insert
    env8 7U J1/13
    Use
    Tags
    GFPuv
    Expression
    Bacterial
    Mutation
    J1/13 converted to 7 U's
    Promoter
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    99829-plasmid-map-sequence-id-192120

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Showing: 421 - 440 of 10438 results