We narrowed to 568 results for: prime editing
-
Plasmid#195512PurposeLentiviral expression plasmid of hMLH1dn with P2A-HygroR markerDepositorInserthMLH1dn-P2A-HygroR (MLH1 Human)
UseLentiviralTagsP2AExpressionMammalianMutationHuman MLH1 del754-756PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MultiMate-Cell-cycle
Plasmid#206280PurposeExpression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.DepositorInsertH2B iRFP713, mAG-hGeminin, mKO2-hCdt1
UseRecombinant baculovirus productionExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-p3-PE(1153-dRnH)-C-term
Plasmid#187182Purposep3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1DepositorInsertPE2 from plasmid #132775 with shortened RnaseH domain
UseAAVAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-HEK3_del1-5-NGG
Plasmid#185478PurposepegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.DepositorInsertHEK3_del1-5-NGG pegRNA
ExpressionMammalianPromoterU6Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-T790M-dual-epegRNA
Plasmid#214100PurposeLentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.DepositorInsertEGFR L858R epegRNA/EGFR T790M epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMDS DEST
Plasmid#206264PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.DepositorTypeEmpty backboneUseCre-donor, multimate/gateway dest vector (multiba…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV EYFP Tubulin
Plasmid#206254PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.DepositorInsertEYFP Tubulin
UseMultimate/gateway entr 1TagsEYFPExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 CMV mTFP1 Actin
Plasmid#206255PurposeENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.DepositorInsertmTFP1 Actin
UseMultimate/gateway entr 2TagsmTFP1ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 CMV mito mCherry
Plasmid#206256PurposeENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.DepositorInsertmito mCherry
UseMultimate/gateway entr 3TagsCOX8 mitochondrial tagExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_KCNA1-mcherry
Plasmid#159788PurposeS. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 geneDepositorInsertspacer of sgRNA targeting KCNA1 gene (KCNA1 Human)
ExpressionMammalianAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-T790M-dual-nick sgRNA
Plasmid#214101PurposeLentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.DepositorInsertEGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD (TET+CTD)
Plasmid#214082PurposeMammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53DepositorAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 hU6 ACTB sgRNA
Plasmid#206270PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNADepositorInsertACTB sgRNA
UseCRISPR; Multimate/gateway entr 2ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV eGFP
Plasmid#206272PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoterDepositorInserteGFP
UseMultimate/gateway entr 4ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 2 hU6 HEK3 PegRNA
Plasmid#206277PurposeENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNADepositorInsertHEK3 PegRNA
UseCRISPR; Multimate/gateway entr 2ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 3 polH aeBlue polH VSV-G
Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3ExpressionBacterial and InsectAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV mTagBFP
Plasmid#206279PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.DepositorInsertmTagBFP
UseMultimate/gateway entr 4ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-21a-PE2-His
Plasmid#201973PurposeExpress His-tagged PE2 in bacteriaDepositorInsertPE2-HIS
TagsHis and c-myc NLSExpressionBacterialAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-PE iSM
Plasmid#235995PurposeExpresses iSM (iSpyMac) prime editor and pegRNA in mammalian cellsDepositorInsertiSM_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsbpSV40 NLSExpressionMammalianAvailable SinceAug. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-N
Plasmid#214734Purposeencodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoterDepositorInsertCAG_iPE-N_bGH
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only