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We narrowed to 23,361 results for: Nov;

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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 human cofilin S3E

Plasmid

#50861

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

Plasmid

#113888

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2

Plasmid

#85208

Purpose

Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1_WT RAD23B

Plasmid

#201457

Purpose

Expresses human RAD23B with an N-terminal GST tag in E. coli

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

tac promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Use

Tags

3xFLAG and GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pScRad52-SpCas9 (pXK-1646)

Plasmid

#208823

Purpose

CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

Depositor

Kun Xu

Article

Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

Type

Empty backbone

Use

CRISPR; Cas9

Tags

Rad52

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-lck-smV5-4x6T

Plasmid

#196419

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox; Astrocyte-selective

Tags

Lck

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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phROSA26-Tet-HA-JLP_WT

Plasmid

#208770

Purpose

Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus

Depositor

Katsuji Yoshioka

Article

Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

Insert

JLP (SPAG9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pWAP-HGF

Plasmid

#83503

Purpose

Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

Depositor

Lothar Hennighausen, Marta Gallego

Article

Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

Insert

HGF (Hgf Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

also contains beta-globin sequences from pUC198

Promoter

mouse WAP (whey acidic protein)

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDRM98 mKate2 Blast

Plasmid

#174720

Purpose

Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

mKate2
BSD

Use

Lentiviral

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Mutation

Promoter

MND and MND (off mKate-P2A)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

Plasmid

#156445

Purpose

For transient expression of mouse RAD21 (SCC1) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse RAD21 (Rad21 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMK425 (GAL-OsTIR1(F74A))

Plasmid

#140657

Purpose

GAL-OsTIR1(F74A)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74A)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCdOpt-BMX

Plasmid

#203929

Purpose

This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

Depositor

Scott Briggs

Article

Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

Insert

BleMX

Use

Tags

Expression

Yeast

Mutation

BleMX was codon optimized for Candida albicans or…

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2

Plasmid

#27415

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
CLXSN 5myc-CDC6-WT

Plasmid

#109332

Purpose

express tagged Cdc6 WT

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL3

Plasmid

#164984

Purpose

NPRL3 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL3 (NPRL3 Human)

Use

Tags

2xSTREP FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF1A-Rigor

Plasmid

#172207

Purpose

Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 251 to Alanine

Promoter

CMV

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-FLCN

Plasmid

#72291

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1011-luc2

Plasmid

#227106

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1011

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_AR-RE_MLPmin_BC1014-luc2

Plasmid

#227108

Purpose

Androgen receptor response element for luciferase and barcode assays; barcode BC1014

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shSMAD4 #1

Plasmid

#83089

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4, Smad4 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCAG/ATP8B4-HA

Plasmid

#209212

Purpose

Mammalian expression of ATP8B4

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B4 (ATP8B4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pME-nls-sfGFP

Plasmid

#132973

Purpose

full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

nuclear localized super folder GFP

Use

Gateway vector

Tags

6x myc and SV40 NLS

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHSN401

Plasmid

#50588

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

3×FLAG-NLS-zCas9-NLS
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Expression

Mutation

maize codon optimized

Promoter

2×35Sp and AtU6-26p

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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