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pTRE-T2-miR-PURO

Plasmid

#102646

Purpose

Tet inducible microRNA and cDNA expression

Depositor

Michael E. Wright

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Promoter

Tet/CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRGD-KmR

Plasmid

#74108

Purpose

Plasmid containing kanamycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aphII

Use

Bacterial vector based on pmb1 replicon

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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SpRY-PCE(cm24Cre)

Plasmid

#242426

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

SpRY-PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_VSBV_G

Plasmid

#207312

Purpose

Mammalian expression of Variegated Squirrel Bornavirus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSBV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_LCMV_GPC

Plasmid

#207288

Purpose

Mammalian expression of Lymphocytic choriomeningitis virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

LCMV_GPC

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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vDip-C: pAAV-Pcp2-mGreenLantern-KASH

Plasmid

#207613

Purpose

vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

Depositor

Longzhi Tan

Article

Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

Insert

mGreenLantern

Use

AAV

Tags

KASH

Promoter

mouse Pcp2 promoter (L7-6)

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP205-AAV-TRE3G-GFP-pA

Plasmid

#164789

Purpose

AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab3a

Plasmid

#170864

Purpose

Mammalian expression of dGFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-UL32

Plasmid

#162664

Purpose

Lentiviral vector for stable expression of UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pREVTRE-AID

Plasmid

#121067

Purpose

Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio

Depositor

Randy Poon

Article

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Use

Retroviral

Tags

AID

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB

Plasmid

#114423

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-TAPP1-PH

Plasmid

#67675

Purpose

Multisite gateway entry clone for adding C-terminal fusions of TAPP1-PH

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

TAPP1-PH

Use

Gateway multisite 3' entry clone

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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B2395_CIBN-BTB-IDR-FRB

Plasmid

#215107

Purpose

Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CIBN-BTB-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-miR-21 toehold switch

Plasmid

#184756

Purpose

Expresses miR-21 sensor with RFP output

Depositor

Allen Liu

Article

Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

Insert

Red Fluorescence Protein

Tags

miR-21 toehold switch

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-IREShygro2

Plasmid

#153966

Purpose

Expresses EGFP in mammalian cells

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

EGFP

Tags

N/A

Expression

Mammalian

Mutation

No mutation

Promoter

CMV promoter

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-CAHS3

Plasmid

#90031

Purpose

To express GFP-tagged CAHS3 in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS3

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDBD-M

Plasmid

#50572

Purpose

Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin

Expression

Yeast

Promoter

Gal1

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
Untagged TDP-43

Plasmid

#237551

Purpose

Expression of untagged wild-type TDP-43

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al Nat Cell Biol. 2026 Mar 18. doi: 10.1038

Insert

TDP-43 WT (TARDBP Human)

Expression

Mammalian

Mutation

wild-type TDP-43

Promoter

CMV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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