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We narrowed to 14,273 results for: cas9

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Showing: 4381 - 4400 of 14273 results

  1. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102885-plasmid-map-sequence-id-259794

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  2. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102889
    Purpose
    PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102889-plasmid-map-sequence-id-198247

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  9. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

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  16. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  19. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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Showing: 4381 - 4400 of 14273 results