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pBEL2296

Plasmid

#164601

Purpose

Complementation plasmid for MlaD (A20F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala20Phe)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2294

Plasmid

#164599

Purpose

Complementation plasmid for MlaD (F13A)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Phe13Ala)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2290

Plasmid

#164596

Purpose

Complementation plasmid for MlaD (G11F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Gly11Phe)-MlaC-MlaB

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pheT

Plasmid

#154920

Purpose

aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pheT

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-mqo

Plasmid

#154921

Purpose

aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting mqo

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisF

Plasmid

#154922

Purpose

aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting hisF

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ampG

Plasmid

#154923

Purpose

aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting ampG

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1647

Plasmid

#155148

Purpose

Expresses MlaF 1-246, tagless

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)

Promoter

araBAD

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-09

Plasmid

#158437

Purpose

pBAD33 vector encoding MotA, Dendra2 - GSGSGS linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

Dendra2- 3 x EAAAK linker

Expression

Bacterial

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-25

Plasmid

#158436

Purpose

pBAD33 vector encoding MotA, YPet - 3 x EAAAK linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

yPET - 3 x EAAAK linker

Expression

Bacterial

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-05

Plasmid

#158435

Purpose

pBAD33 vector encoding MotA, YPet - EAAAK linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Tags

yPET - EAAAK linker

Expression

Bacterial

Available Since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1889

Plasmid

#139893

Purpose

Complementation plasmid for LetAB with LetB PLL4 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta459-360)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2071

Plasmid

#139928

Purpose

Complementation plasmid for LetAB operon with LetA and LetB genes deoverlapped

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB_deoverlapped

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1859

Plasmid

#139906

Purpose

Complementation plasmid for LetAB with LetB mutant V247N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(V247N)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1858

Plasmid

#139905

Purpose

Complementation plasmid for LetAB with LetB mutant L246N

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(L246N)

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1731

Plasmid

#139922

Purpose

Expression plasmid for LetB mutant E854Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; E854Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1730

Plasmid

#139921

Purpose

Expression plasmid for LetB mutant K488Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; K488Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1729

Plasmid

#139920

Purpose

Expression plasmid for LetB mutant F833Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F833Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1728

Plasmid

#139919

Purpose

Expression plasmid for LetB mutant F818Bpa

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; F818Amber)-6xHis

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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