We narrowed to 7,766 results for: loxP
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Plasmid#160994PurposeExpress mouse p150 protein with PIP2 deletion mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHAF1A (Chaf1a Mouse)
TagsGFPExpressionMammalianMutationPIP2 motif deletionAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE
Plasmid#131730PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strainDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTagBFP2-V5 tagExpressionMammalianMutationG12VPromoternone (4x polyA to mitigate episomal expression)Available sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-IRES-EGFP
Plasmid#153949PurposepiggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFPDepositorAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR
Plasmid#137733PurposeLentivirus vector for TLR2/4 miR in a Cre-dependent mannerDepositorInsertTKR2/4 microRNA
UseLentiviralTagsmChherryExpressionMammalianAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNAs[bTub+Sxl].1046B
Plasmid#112692Purposeexpress two gRNA targeting bTub & Sxl under dU6-3 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
UseCRISPRAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Desmoplakin II tension sensor (FL-based)
Plasmid#118721PurposeThe FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II no force control (F40-based)
Plasmid#118716PurposeThe no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-F40-mCherryExpressionMammalianMutationtruncation after aa1353PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-ADCY6-C2 (917-1165 aa)
Plasmid#113904PurposeHis-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
TagsHis-SExpressionBacterial, Insect, and Mamm…MutationPlease see Depositor CommentsPromoterT7, CMVAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-ADCY6-C1(306-672 aa)
Plasmid#113903PurposeHis-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
TagsHis-SExpressionBacterial, Insect, and Mamm…PromoterT7, CMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC- IKZF5-3xHA-pGK-PUR
Plasmid#107385PurposeLentiviral expression of IKZF5-3xHADepositorAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-Flag-dTSC2 WT
Plasmid#111807PurposeInsect Expression of dTSC2DepositorAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE
Plasmid#111387PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)DepositorAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE
Plasmid#111388PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)DepositorAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
b2AR->M71-IRES-tauGFP-ACNF TV
Plasmid#105198Purposetargeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseMouse TargetingAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-FLAG-YWAA-pGK-HYG
Plasmid#107375PurposeLentiviral expression of FLAG-YWAADepositorInsertcereblon (CRBN Human)
UseLentiviralTagsFLAGExpressionMammalianMutationCRBN-Y383A/W385APromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX398 Nitratifractor salsuginis str DSM 16511 Cas9
Plasmid#68334PurposeNitratifractor salsuginis str DSM 16511 Cas9DepositorInsertNsCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWPT-UCU/ACC-mEGFP-IRES-mCherry
Plasmid#49232PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-TIR1-HA
Plasmid#172610PurposeLentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cellsDepositorInsertTIR1
UseLentiviralTagsHAExpressionMammalianPromoterTet/ON Minimal CMV promoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -