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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 4401 - 4420 of 11938 results

  6. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Publication
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  9. pMito-dCherry-FRB

    Plasmid
    #186573
    Purpose
    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
    Depositor
    Stephen Royle
    Publication
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dark MitoTrap
    Expression
    Mammalian
    Mutation
    Mutation of "K70N" to render mCherry no…
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    186573-plasmid-map-sequence-id-368442

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  10. Man2a1-myc

    Plasmid
    #163648
    Purpose
    Expresses myc tagged Man2a1 in mammalian cells
    Depositor
    Lei Lu
    Publication
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    mannosidase alpha class 2A member 1 (MAN2A1 Human)
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Silent mutation was made to introduce a XbaI rest…
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163648-plasmid-map-sequence-id-322675

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  12. UAS:bloSwitch

    Plasmid
    #108355
    Purpose
    UAS driven green to red switch reporter for B3 recombinase expression
    Depositor
    Harold Burgess
    Publication
    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.
    Insert
    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    Use
    Zebrafish expression
    Mutation
    Zebrafish codon optimized
    Promoter
    lac
    Available since
    Sept. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    108355-plasmid-map-sequence-id-208272

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  13. pIRES:mTREK-1(G137I)

    Plasmid
    #133274
    Purpose
    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag
    Depositor
    Dan Minor
    Publication
    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G137I
    Promoter
    CMVd2
    Available since
    April 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    133274-plasmid-map-sequence-id-292435

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Showing: 4401 - 4420 of 11938 results