Addgene

We narrowed to 23,361 results for: Nov;

Showing: 6631 - 6660 of 23361 results

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pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

Plasmid

#156436

Purpose

For transient expression of human PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5B (PDS5B Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLuc.mKO2

Plasmid

#85224

Purpose

shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

Firefly Luciferase

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Use

Tags

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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EF1a_LMX1A_P2A_Hygro_Barcode

Plasmid

#120457

Purpose

Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

LMX1A (LMX1A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pICSL11056

Plasmid

#68258

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN

Plasmid

#72290

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN (FLCN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
EF1a_FOXA3_P2A_Hygro_Barcode

Plasmid

#120440

Purpose

Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA3 (FOXA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
psigma2-EGFP

Plasmid

#53610

Purpose

Expresses sigma2 of adaptor protein of AP2

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma2 (Ap2s1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-TAF15N-mCherry-sspB

Plasmid

#122436

Purpose

Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

mCherry and sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA1_P2A_Hygro_Barcode

Plasmid

#120438

Purpose

Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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INPP5D-eGFP WT

Plasmid

#161998

Purpose

Mammalian expression vector, containing a GFP tagged version of INPP5D

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5D (INPP5D Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE6.3

Plasmid

#102916

Purpose

expresses ABE6.3 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE6.3

Use

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13

Plasmid

#157712

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LacI-11/109-pHG165c

Plasmid

#90059

Purpose

Dimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI-11/109

Use

Tags

Expression

Bacterial

Mutation

109

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_hNgn1_UBC_Bla

Plasmid

#61473

Purpose

3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn1 (NEUROG1 Human)
Bla

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-BlastR

Plasmid

#193848

Purpose

Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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xCas9

Plasmid

#138565

Purpose

Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

xCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVd1-EGFP-hRhoA-wt

Plasmid

#133926

Purpose

Low level expression of GFP fused human RhoA wild type

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

GFP-hRhoA WT (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PhLP1-myc-6xHis

Plasmid

#218176

Purpose

Expresses phosducin-like protein 1 in mammalian cells

Depositor

Barry Willardson

Article

Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

Insert

Phosducin like protein 1 (PDCL Human)

Use

Tags

6x His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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LLP788_Traptavidin-EZH2-FL-CO

Plasmid

#211781

Purpose

AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-EZH2

Use

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect EZH2 e…

Promoter

pEF1a and pSV40

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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