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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,416 results for: sequence

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Showing: 4421 - 4440 of 13416 results

  1. T-FAM83BA-6

    Plasmid
    #166551
    Purpose
    scFv of a human scaffold targeting Protein FAM83B. Antigen coverage aa 117-294 of 1011
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166551-plasmid-map-sequence-id-329242

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  2. O-AASSA-25

    Plasmid
    #166537
    Purpose
    scFv of a human scaffold targeting Aminoadipate-semialdehyde synthase. Antigen coverage aa 455-926 of 926
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166537-plasmid-map-sequence-id-328275

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  3. J-CDAN1A-5

    Plasmid
    #166522
    Purpose
    scFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166522-plasmid-map-sequence-id-328268

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  4. Z-OPTN-10

    Plasmid
    #166562
    Purpose
    scFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166562-plasmid-map-sequence-id-327812

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  5. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  6. pLV6-Bmal-luc

    Plasmid
    #68833
    Purpose
    Expression of Bmal1-luc in mammalian cells
    Depositor
    Steven Brown
    Insert
    Bmal1 regulatory sequences (Bmal1 Mouse)
    Use
    Lentiviral and Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Available Since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68833-plasmid-map-sequence-id-455401

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  7. pGFP FAK Y397F

    Plasmid
    #50516
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    protein tyrosine kinase, mutated
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y397F
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

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  11. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  13. pAd/WT1-IRES-nAmCyan

    Plasmid
    #29756
    Depositor
    Edward McCabe
    Insert
    Wilms tumor 1 (WT1 Human)
    Tags
    AmCyan, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; AmCyan is nuclear localized; WT1 an…
    Available Since
    Oct. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    29756-plasmid-map-sequence-id-137669

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  15. O-TREM2-2

    Plasmid
    #166545
    Purpose
    scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166545-plasmid-map-sequence-id-329237

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  16. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

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  17. pLNHA-C1-HseIF4AI_K

    Plasmid
    #146731
    Purpose
    Mammalian Expression of HseIF4AI
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4AI (EIF4A1 Human)
    Expression
    Mammalian
    Mutation
    a non silent mutation (A200V) and 3 silent mutati…
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    146731-plasmid-map-sequence-id-357210

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  18. pT7-V5-SBP-C1-HsUpf1_1-925-siRNAres_P

    Plasmid
    #147191
    Purpose
    Mammalian Expression of HsUpf1_1-925-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1_1-925-siRNAres (UPF1 Human)
    Expression
    Mammalian
    Mutation
    4 non silent mutations A69S, E167G, K282R and A4…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147191-plasmid-map-sequence-id-430435

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  19. pT7-V5-SBP-C1-HsUpf1-del296-926_P

    Plasmid
    #147195
    Purpose
    Mammalian Expression of HsUpf1-del296-926
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-del296-926 (UPF1 Human)
    Expression
    Mammalian
    Mutation
    4 non silent mutations A69S, E167G, K282R and T1…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147195-plasmid-map-sequence-id-430443

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  20. pT7-V5-SBP-C1-HsUpf1_115-1129-siRNAres_P

    Plasmid
    #147193
    Purpose
    Mammalian Expression of HsUpf1_115-1129-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1_115-1129-siRNAres (UPF1 Human)
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Available Since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    147193-plasmid-map-sequence-id-355704

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Showing: 4421 - 4440 of 13416 results