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pRS313 - prp8 T1872E

Plasmid

#90970

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

T1872E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 T1861P

Plasmid

#90971

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

T1861P

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1862Y

Plasmid

#90972

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1862Y

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 H1863E

Plasmid

#90973

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

H1863E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 K1864E

Plasmid

#90974

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

K1864E

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 N1869D

Plasmid

#90975

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

N1869D

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 V1870N

Plasmid

#90976

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

V1870N

Availability

Academic Institutions and Nonprofits only

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pRS313 - prp8 I1875T

Plasmid

#90977

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Mutation

I1875T

Availability

Academic Institutions and Nonprofits only

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pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Article

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCAG-human TMEM217-PA

Plasmid

#249410

Purpose

Expression vector of human TMEM217 isoform 3 tagged with PA at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Transmembrane protein 217 isoform 3 (TMEM217 Human)

Tags

PA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pHBT-KRAS174

Plasmid

#248050

Purpose

To express His6 tag-Avi tag-TEV cleavage site-KRAS (residues 1-174) in E. coli, under the T7 promoter.

Depositor

Shohei Koide

Article

Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

KRAS (KRAS Human)

Tags

His6 tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

includes aa 1-174 only

Promoter

T7

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pEE844

Plasmid

#231167

Purpose

T-DNA encoding TRV2 with PaqCI Recognition Sites and Ccdb

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Type

Empty backbone

Use

Cloning vector

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEE834

Plasmid

#231168

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlPDS

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Insert

mobile gRNA targeting SlPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pC129: pAAV.CMV-CasRx mCh

Plasmid

#203442

Purpose

Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

RfxCas13d-T2A-mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

Plasmid

#203451

Purpose

Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Codon optimised using GenScript tool

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mKate2

Plasmid

#220599

Purpose

Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mKate2

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-AausFP1

Plasmid

#220595

Purpose

Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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RNAseq_cal_Tuschl

Plasmid

#59426

Purpose

Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

Depositor

Thomas Tuschl

Article

Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

Insert

RNAseq_cal_Tuschl

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Luciferase-CD4-bla

Plasmid

#189981

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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