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pPB-cT3G-cERP2-ZGLP1

Plasmid

#222926

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZGLP1 (ZGLP1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-TEV-GFP-NAP1

Plasmid

#208870

Purpose

For expression in E. coli of GFP-NAP1

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

GFP and GST-TEV

Expression

Bacterial

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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SF3B3

Plasmid

#156003

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SF3B3 (SF3B3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

S6L

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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RBMX2-1

Plasmid

#155915

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBMX2 (RBMX2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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PHF6

Plasmid

#155821

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PHF6 (PHF6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-Fzd1mApple

Plasmid

#203795

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

Fzd1 (fzd1 Zebrafish)

Use

Zebrafish expression

Tags

mApple

Promoter

4kb Wnt3 promoter

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one_Bip gRNA1

Plasmid

#183696

Purpose

Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH

Plasmid

#148910

Purpose

Insect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-del331-370-YFWFtoAAAA_AH

Plasmid

#148912

Purpose

Insect Expression of DmGIGYF-del331-370-YFWFtoAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YFWFtoAAAA (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmGIGYF-del331-370-YFWFtoAAAA-dsRNAres_AH

Plasmid

#148927

Purpose

Insect Expression of DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE

Plasmid

#148623

Purpose

Bacterial Expression of DmRoquin_702-819-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-DmRoquin-del725-755-del790-812_AE

Plasmid

#148608

Purpose

Mammalian Expression of DmRoquin-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin-del725-755-del790-812 (roq Fly)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE

Plasmid

#148622

Purpose

Bacterial Expression of DmRoquin_702-819-del725-755

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del725-755 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmRoquin-del725-755-del790-812_AE

Plasmid

#148591

Purpose

Insect Expression of DmRoquin-del725-755-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin-del725-755-del790-812 (roq Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE

Plasmid

#148598

Purpose

Insect Expression of DmNot1_1467-1717

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmNot1_1467-1717 (Not1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-R249H_X

Plasmid

#147979

Purpose

Insect Expression of DmCaf40-R249H

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-R249H (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-Y208AR210AH213AA253F_X

Plasmid

#147980

Purpose

Insect Expression of DmCaf40-Y208AR210AH213AA253F

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-Y208AR210AH213AA253F (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-Y208AR249A_X

Plasmid

#147981

Purpose

Insect Expression of DmCaf40-Y208AR249A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-Y208AR249A (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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