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pAAV-EF1⍺-DIO-Cx35-M1-mApple

Plasmid

#255635

Purpose

Encodes M. americana Cx35, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mApple tag. Mutation of K221E. Expression driven by EF1a promoter.

Depositor

Kafui Dzirasa

Article

Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586

Insert

Cx35

Use

AAV

Tags

mApple

Mutation

K221E

Promoter

EF1a

Available Since

June 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Cx35-M1-mApple

Plasmid

#255637

Purpose

Encodes floxed (DIO) M. americana Cx35, codon optimized for mouse expression and c-terminally fused mApple tag. Mutation of K221E. Expression driven by hSyn promoter.

Depositor

Kafui Dzirasa

Article

Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586

Insert

Cx35

Use

AAV

Tags

mApple

Mutation

K221E

Promoter

hSyn

Available Since

June 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1⍺-DIO-Cx34.7-M1-mEmerald

Plasmid

#255634

Purpose

Encodes M. americana Cx34.7, codon optimized for mouse expression, with 8 amino acid linker and c-terminally fused mEmerald tag. Mutations of E214K and E223K. Expression driven by EF1a promoter.

Depositor

Kafui Dzirasa

Article

Ransey et al Nature. 2026 May 13. doi: 10.1038/s41586

Insert

Cx34.7

Use

AAV

Tags

mEmerald

Mutation

E214K,E223K

Promoter

EF1a

Available Since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-IgK-R-deLACCO1-COBRA

Plasmid

#223344

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

deLACCO1

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-IgK-R-eLACCO2-COBRA

Plasmid

#223345

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-IgK-R-eLACCO2.1-COBRA

Plasmid

#223346

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACC02.1

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-IgK-R-eLACCO2-COBRA

Plasmid

#223348

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-ExRai-AKAR2-T/A

Plasmid

#171847

Purpose

Cre-dependent, synapsin promoter-driven expression of the negative control phosphomutant ExRai-AKAR2 T/A PKA biosensor in neurons.

Depositor

Richard Huganir

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2 T/A

Use

AAV

Expression

Mammalian

Mutation

T6A phospho-deficient mutation

Promoter

hSyn1

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec

Plasmid

#239596

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

Plasmid

#239594

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

Plasmid

#239595

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pC130: pAAV.CMV-CasRx-VEGFA sgRNA

Plasmid

#203443

Purpose

Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

Plasmid

#203447

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

Plasmid

#227800

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 2 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

Plasmid

#227801

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA 3 (Vegfa Synthetic)
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Expression

Bacterial and Mammalian

Promoter

hSyn1

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hsChRmine-oScarlet-WPRE

Plasmid

#183520

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

hsChRmine-oScarlet

Use

AAV

Mutation

H33R

Promoter

CaMKIIa

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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AiP14454: pAAV-AiE0783m-minBG-SYFP2-WPRE3-BarcodeL-BGHpA (Alias: CN4454)

Plasmid

#236593

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0783m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14453: pAAV-AiE0441h-minBG-SYFP2-WPRE3-BarcodeK-BGHpA (Alias: CN4453)

Plasmid

#236592

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0441h is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14449: pAAV-AiE0367h_3xC2-minRho-SYFP2-WPRE3-BarcodeG-BGHpA (Alias: CN4449)

Plasmid

#236588

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0367h_3xC2 is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14461: pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BarcodeS-BGHpA (Alias: CN4461)

Plasmid

#236600

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0779m_3xC2 is an enhancer active in striatal D1-MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14458: pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BarcodeP-BGHpA (Alias: CN4458)

Plasmid

#236597

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0441h_3xC2 is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14456: pAAV-AiE0603m-minBG-SYFP2-WPRE3-BarcodeN-BGHpA (Alias: CN4456)

Plasmid

#236595

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0603m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13401: pAAV-AiE0140h-minBG-FlpO-4X2C-WPRE3-BGHpA (Alias: CN3401)

Plasmid

#236585

Purpose

AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and hippocampal Pvalb neurons

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

AiE0140h-minBG-FlpO-4X2C

Use

AAV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14459: pAAV-AiE0785m-minBG-SYFP2-WPRE3-BarcodeQ-BGHpA (Alias: CN4459)

Plasmid

#236598

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0785m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP14457: pAAV-AiE0784m-minBG-SYFP2-WPRE3-BarcodeO-BGHpA (Alias: CN4457)

Plasmid

#236596

Purpose

Barcoded enhancer AAV vector for multiplexed screening applications; AiE0784m is an enhancer active in striatal MSNs

Depositor

Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge