We narrowed to 14,198 results for: egf

1. pCLJ-Fgf5-t2A-eGFP

   Plasmid

   #227504

   Purpose

   Inserts t2a-eGFP at the 3' end of the Fgf5 gene alsong with a removable selection cassette

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1 EGFP-p62 HRD

   Plasmid

   #207550

   Purpose

   Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible EGFP-P62
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP-EGFP-PA-BAX(lyso)

   Plasmid

   #228939

   Purpose

   Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   TMEM106B (TMEM106B Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Promoter

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-EGFP-LOV2-OMP25

   Plasmid

   #233346

   Purpose

   Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Omp25 (Synj2bp Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted and N538E

   Promoter

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP-EGFP-LOV2-TMEM106B

   Plasmid

   #233347

   Purpose

   Stable expression of EGFP-LOV2(G528A, N538E)-TMEM106B in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   TMEM106B (TMEM106B Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted (TMEM106B 90-274) and N538E

   Promoter

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIP-LAMP1-EGFP-APEX2-V5

   Plasmid

   #236402

   Purpose

   Expresses a fluorescent lysosome-localized APEX2

   Depositor

   Jared Rutter

   Article

   Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

   Insert

   LAMP1-EGFP-APEX2-V5
   

   Use

   Retroviral

   Tags

   APEX2, EGFP, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIP-EGFP-APEX2-V5-EEA1

   Plasmid

   #236403

   Purpose

   Expresses a fluorescent endosome-localized APEX2

   Depositor

   Jared Rutter

   Article

   Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

   Insert

   EGFP-APEX2-V5-EEA1
   

   Use

   Retroviral

   Tags

   APEX2, EGFP, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQCXIP-Pex3-EGFP-APEX2-V5

   Plasmid

   #236401

   Purpose

   Expresses a fluorescent peroxisome-localized APEX2

   Depositor

   Jared Rutter

   Article

   Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

   Insert

   Pex3(fragment)-EGFP-APEX2-V5
   

   Use

   Retroviral

   Tags

   APEX2, EGFP, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQCXIP-EGFP-ADRP-APEX2-V5

   Plasmid

   #236405

   Purpose

   Expresses a fluorescent lipid droplet-localized APEX2

   Depositor

   Jared Rutter

   Article

   Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

   Insert

   EGFP-ADRP-APEX2-V5
   

   Use

   Retroviral

   Tags

   APEX2, EGFP, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-EGFP/VSVG-rbPodxl

    Plasmid

    #233260

    Purpose

    Expression of EGFP-tagged PODXL WT

    Depositor

    Keith Mostov, David Bryant

    Article

    Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

    Insert

    PODXL (PODXL Rabbit)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-MTS(S17A)-EGFP-V5

    Plasmid

    #200928

    Purpose

    expresses MTS(S17A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S17A in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-MTS(S23A)-EGFP-V5

    Plasmid

    #200929

    Purpose

    expresses MTS(S23A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    MTS amino acid 23 mutated to Alanine

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS-MTS(S27A)-EGFP-V5

    Plasmid

    #200930

    Purpose

    expresses MTS(S27A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S27A in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-MTS(S31A)-EGFP-V5

    Plasmid

    #200931

    Purpose

    expresses MTS(S31A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S31A in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-MTS(S34A)-EGFP-V5

    Plasmid

    #200932

    Purpose

    expresses MTS(S34A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S34A in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS--MTS(S43A)-EGFP-V5

    Plasmid

    #200933

    Purpose

    expresses MTS(S43A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S43A in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-MTS(S6A)-EGFP-V5

    Plasmid

    #200817

    Purpose

    expresses MTS(S6A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    Changed Serine 6 to Alanine in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS-MTS(S12A)-EGFP-V5

    Plasmid

    #200818

    Purpose

    expresses MTS(S12A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S12A in MTS

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid

    #177424

    Purpose

    To express Venus fused to Noxa-4E, with a longer linker sequence between compared to Addgene #166745.

    Depositor

    David Andrews

    Insert

    Noxa, PMA-induced protein 1 (PMAIP1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

    Plasmid

    #233368

    Purpose

    hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_on-mEGFP
    

    Use

    AAV

    Tags

    NES and mEGFP

    Expression

    Mutation

    Promoter

    hSyn1

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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