We narrowed to 9,853 results for: otos

1. gCOTS-pyl

   Plasmid

   #92048

   Purpose

   This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Cyanobacteria s. elongatus genome recombination i…

   Tags

   Expression

   Mutation

   Promoter

   LeuP (native S. elongatus promoter) and PrcbL

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ING5

   Plasmid

   #13290

   Depositor

   Curtis Harris

   Article

   Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

   Insert

   ING5 (ING5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pBS-TRE-mTagBFP2-WPRE

   Plasmid

   #193332

   Purpose

   Expresses mTagBFP2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A XhoI site in the coding sequence is silenced

   Promoter

   TRE

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPlum-Gamma-Tubulin-17

   Plasmid

   #55971

   Purpose

   Localization: Centrosomes, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. MAC-SARS-CoV-2 Orf7b

   Plasmid

   #158376

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 Orf7b (ORF7b )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pMGS59(AID-ARF-P2A-Hygromycin)

   Plasmid

   #138174

   Purpose

   An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

   Insert

   AID-ARF-P2A-Hygromycin
   

   Use

   Tags

   AID-ARF clamp degron tag

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC-SARS-CoV-2 NSP15

   Plasmid

   #158366

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP15 (ORF1ab )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET28a Gx-NL3

   Plasmid

   #169108

   Purpose

   Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSM12

   Plasmid

   #101643

   Purpose

   Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   meffCFP
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCerulean3-EB1-C-18

    Plasmid

    #55415

    Purpose

    Localization: MT End Binding Protein, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-P1-N-miRFP670-C

    Plasmid

    #159438

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-P1-N-EGFP-C

    Plasmid

    #159436

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSM17

    Plasmid

    #101641

    Purpose

    Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    eqFP486
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC-SARS-CoV-2 NSP7

    Plasmid

    #158358

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP7 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. EYFP-Clathrin-19

    Plasmid

    #56584

    Purpose

    Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pJL1-EPA-DNNNS-DQNRT

    Plasmid

    #128397

    Purpose

    In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-Nxph4-6A-HA

    Plasmid

    #128103

    Purpose

    Express HA tagged Nxph4-6A

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mVenus-CENPB-N-22

    Plasmid

    #56325

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. TRIN-E-shREN

    Plasmid

    #105585

    Purpose

    Dox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    control shRNA targeting luciferase
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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