We narrowed to 33,660 results for: IND

1. pcDNA5-hygro-eGFP-STOP-HEC1

   Plasmid

   #114026

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-STOP-HEC1 (NDC80 Human)
   

   Expression

   Mammalian

   Mutation

   Start codon of Hec1 is mutated to stop codon

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111922

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PatoK-OG241

   Plasmid

   #72840

   Purpose

   NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoK
   

   Expression

   Bacterial

   Promoter

   PatoK

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PatoL-LOG241

   Plasmid

   #72846

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Expression

   Bacterial

   Promoter

   PatoL

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PatoK-LOG241

   Plasmid

   #72848

   Purpose

   NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoK
   

   Expression

   Bacterial

   Promoter

   PatoK

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET151-GFP-PP-RPN2 (918-953)

   Plasmid

   #73743

   Purpose

   Expression of GFP-PP-RPN2 (918-53) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 918-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pET151-GFP-PP-RPN2 (932-953)

   Plasmid

   #73745

   Purpose

   Expression of GFP-PP-RPN2 (932-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 932-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pET151-GFP-PP-RPN2 (918-947)

    Plasmid

    #73747

    Purpose

    Expression of GFP-PP-RPN2 (918-947) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-947 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pET151-GFP-PP-RPN2 (797-953)

    Plasmid

    #74228

    Purpose

    [Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-953 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

    Plasmid

    #83462

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, W4107A

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

    Plasmid

    #83460

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, S4144D

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

    Plasmid

    #83459

    Purpose

    Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, S4144D

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

    Plasmid

    #83457

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET151-hUCH37 (ISF1)(M148D/F149D)

    Plasmid

    #71388

    Purpose

    Expression of hUCH37 (M148D/F149D) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
    

    Tags

    HIS-TEV sequence

    Expression

    Bacterial

    Mutation

    changed methionine 148 to aspartate, phenylalanin…

    Promoter

    T7

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5FRT/TO-GFP-I3(V41A/W43A)

    Plasmid

    #61402

    Purpose

    Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    inhibitor-3 (PPP1R11 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V41A/W43A

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pHL1529

    Plasmid

    #53027

    Purpose

    PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    GFP
    tetR
    csrA
    

    Use

    Synthetic Biology

    Tags

    RBS(st2), RBS(st3), and glgC leader contains -61 …

    Expression

    Bacterial

    Promoter

    pConNoHind and pLlac0-1

    Available Since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pHcRed-NPM1c-C1

    Plasmid

    #131821

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is the cytoplasmic mutant causing AML, where…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid

    #192453

    Purpose

    intronized ttLbCas12a for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Mutation

    temperature-tolerant mutation (D156R)

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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