We narrowed to 8,837 results for: Mela

1. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF1-MCS2-EF1-Puro-RGS7-E383K

   Plasmid

   #113731

   Purpose

   Express mutant RGS7- E383K- in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E383K

   Promoter

   CMV

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF1-MCS2-EF1-Puro-RGS7-R44C

   Plasmid

   #113729

   Purpose

   Express mutant RGS7- R44C- in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF1-R310_RASA2-EF1-Puro

   Plasmid

   #114097

   Purpose

   Express mutant RASA2 in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

   Insert

   RASA2 (RASA2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R310*

   Promoter

   CMV

   Available Since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

   Plasmid

   #112987

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   S123-125-3A

   Promoter

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

   Plasmid

   #112985

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   S123-125-3D

   Promoter

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL c2x Tral FL-15D

   Plasmid

   #113010

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to D

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc-GFP-dSUMOdeltaC

   Plasmid

   #85697

   Purpose

   expression of GFP-SUMO1 delta C in Drosophila cells

   Depositor

   Giannino Del Sal, Licio Collavin

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   SUMO (Sumo Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   non-conjugatable SUMO, lacking the C-terminal gly…

   Promoter

   pAc5

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

10. KHBD00320

    Plasmid

    #34445

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6376 (E2f1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid

    #126473

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

    Plasmid

    #128278

    Purpose

    Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

    Depositor

    Anna Sokac

    Article

    Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

    Insert

    twinstar (tsr) (tsr Fly)
    

    Use

    Tags

    6X Histidine tag

    Expression

    Bacterial

    Mutation

    Met1 changed to Pro1; Cys77 silent basepair mutat…

    Promoter

    T7

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid

    #126472

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-hygro-2xMyc-Rac1-P29S

    Plasmid

    #128581

    Purpose

    For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Mutation

    Changed Proline29 to Serine.

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pUCIDT-attL1-Human ABeta-attR5

    Plasmid

    #160436

    Purpose

    Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-ATAD2-plx303

    Plasmid

    #188635

    Purpose

    ATAD2 overexpression

    Depositor

    Lorenz Studer

    Article

    Baggiolini et al Science. 2021 Sep 3;373(6559):eabc1048.

    Insert

    ATAD2 (ATAD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-CASANOVA

    Plasmid

    #113036

    Purpose

    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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