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pMal-EcPhr

Plasmid

#136244

Purpose

Protein expression of E. coli Phr

Depositor

Aziz Sancar

Article

Selby et al Proc Natl Acad Sci U S A. 2006 Nov 21. 1

Insert

EcPhr

Tags

MBP

Expression

Bacterial

Mutation

V115E- Please see depositor comments below

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Pado Red pcDNA3.1

Plasmid

#79665

Purpose

Pado with mKate2

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado mKate2

Expression

Mammalian

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pQE-WT-N4-R5-YRNRV

Plasmid

#63625

Purpose

N4-R5-YRNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-YRNRV RNAP

Mutation

N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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ZE3 Wnt-8

Plasmid

#17044

Depositor

Randall Moon

Article

Kelly et al Development. 1995 Jun. 121(6):1787-99.

Insert

wnt-8 full length (wnt8a Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pRubiC-T2A-cre

Plasmid

#66692

Purpose

Recombine floxed alleles in newborn neurons

Depositor

Bryan Luikart

Article

Williams et al J Neurosci. 2015 Jan 21;35(3):943-59. do

Insert

mCherry-T2A-Cre

Use

Retroviral

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDA12

Plasmid

#113663

Purpose

Cloning vector for bacterial expression with Pdhfr promoter

Depositor

Miguel Valvano

Article

Aubert et al Infect Immun. 2008 May;76(5):1979-91. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pdhfr

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCherry-ABCC1

Plasmid

#34942

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC1

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
Pado pcDNA3.1

Plasmid

#79663

Purpose

Proton Channel Fluorescent Voltage Indicator

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado Green

Expression

Mammalian

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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HeatShock-acNPV_Bxb1_SV40UTR

Plasmid

#87641

Purpose

For creating drosophila transgenics expressing HeatShock-acNPV_Bxb1_SV40UTR

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR

Plasmid

#87642

Purpose

For creating drosophila transgenics expressing HeatShock-Hsp70Bb_Bxb1_Hsp70BbUTR

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCherry-ABCG2a

Plasmid

#34943

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCG2a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pGEXndoE

Plasmid

#47714

Purpose

Expresses GST-EndoE from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Tags

Glutathione S-transferase

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-LA::Halo2

Plasmid

#87651

Purpose

For creating drosophila transgenics expressing UAS-LA::Halo2

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

LA::Halo2

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pWJPrP43

Plasmid

#22112

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP ki-3F4-FFI TC

Use

Mouse Targeting; Construction of targeting vector

Mutation

3F4 epitope and FFI related D177N mutation

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
sCAG-DIO-oScarlet3

Plasmid

#247721

Purpose

Used for AAV1 tracing experiment.

Depositor

Boxuan Zhao

Article

Chen et al bioRxiv [Preprint]. 2025 May 26:2025.02.

Insert

oScarlet3

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist_21tRNAs_split

Plasmid

#246822

Purpose

Plasmid for simultaneous expression of the minimum 21 tRNA set

Depositor

Norikazu Ichihashi

Article

Miyachi et al Nat Commun. 2025 Aug 26;16(1):7418. doi:

Insert

E. coli tRNA arrays

Expression

Bacterial

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET-C5

Plasmid

#246823

Purpose

Expression plasmid for C5 protein of E. coli RNase P

Depositor

Norikazu Ichihashi, Takashi Yokogawa

Article

Miyachi et al Nat Commun. 2025 Aug 26;16(1):7418. doi:

Insert

C5 protein of E. coli RNase P

Tags

Histidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5427

Plasmid

#229034

Purpose

tetO-phi29 DNA polymerase ‘dead’ reporter: PT7-tetO-B0035-p2 D249E-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-RBS35-phi29 DNAP (D249E, dead)-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5451

Plasmid

#231066

Purpose

tetO-10xHis-phi29 DNA polymerase PovSak reporter: PT7-tetO-B0035-10xHis-p2 povsak-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-B0035-10xHis-phi29 DNAP PovSak-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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