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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27,479 results for: cas9

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Showing: 4481 - 4500 of 27479 results

  3. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid
    #98973
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 2 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98973-plasmid-map-sequence-id-199215

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  7. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid
    #178090
    Purpose
    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Publication
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    178090-plasmid-map-sequence-id-350140

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  8. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    175570-plasmid-map-sequence-id-344992

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  10. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  11. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Publication
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  12. pcDNA3-His:dCas9:nls:10P3

    Plasmid
    #135982
    Purpose
    Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9 fused to 10 copies of the P3 coiled-coil
    Use
    CRISPR
    Tags
    His
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135982-plasmid-map-sequence-id-308932

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  13. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

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Showing: 4481 - 4500 of 27479 results