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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,359 results for: 11

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Showing: 4541 - 4560 of 8359 results

  1. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Publication
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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  2. pCAG-RFP-p2a-postASAP

    Plasmid
    #178793
    Purpose
    Expresses voltage sensor targeted to dendrites and spines along with RFP
    Depositor
    Rafael Yuste
    Publication
    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FingR.PSD95, ASAP2f and TagRFP
    Tags
    TagRFP
    Expression
    Mammalian
    Mutation
    mutations in amino acids L146G, S147T N149R, S150…
    Promoter
    CAG
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    178793-plasmid-map-sequence-id-359665

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  6. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128587
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α (1.1 kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128587-plasmid-map-sequence-id-257676

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  7. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid
    #114077
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R/S542R/K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114077-plasmid-map-sequence-id-220525

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  8. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid
    #114075
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114075-plasmid-map-sequence-id-220517

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  9. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106179
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106179-plasmid-map-sequence-id-204124

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  10. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106190
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106190-plasmid-map-sequence-id-204216

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  16. pYO395

    Plasmid
    #235734
    Purpose
    Expression of human VPS34 complex I in mammalian cells
    Depositor
    Roger Williams
    Publication
    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ATG14 (ATG14 Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    Tags
    ZZ-3xTEV
    Expression
    Mammalian
    Mutation
    N256S
    Promoter
    CMV
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    235734-plasmid-map-sequence-id-471647

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  18. pTon1(acgfp1)

    Plasmid
    #233648
    Purpose
    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.
    Depositor
    Takeo Kubo
    Publication
    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    Use
    Amphibian expression, tet-on
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233648-plasmid-map-sequence-id-468211

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Showing: 4541 - 4560 of 8359 results