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pXPR_003 sgGPAA1 guide 2

Plasmid

#193609

Purpose

GPAA1 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgGPAA1 guide 2 (GPAA1 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

Plasmid

#191481

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR ΔSL1

Use

Lentiviral and Luciferase

Mutation

Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMA5460

Plasmid

#184855

Purpose

Encodes G418 resistance and PhiC31-excisable hTFAM gene

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM (TFAM Human)

Use

Retroviral; Phic31 attp-attb recombination/excisi…

Expression

Mammalian

Mutation

WT

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMA4000

Plasmid

#184857

Purpose

Cre-excisable TFAM in Blasticidin-resistannce retroviral vector

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM (TFAM Human)

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pk_P38-Cd4-bio-His

Plasmid

#126846

Purpose

Expression of recombinant P. knowlesi protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P38 (PKH_102490 Plasmodium knowlesi)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_P12-Cd4-bio-His

Plasmid

#126822

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P12 (P12p Plasmodium malariae)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-nTEVp

Plasmid

#179670

Purpose

Mutant Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

nTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-cTEVp

Plasmid

#179671

Purpose

Mutant Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Tags

cTEVP

Expression

Mammalian

Mutation

K65E, R89L

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-mut1_G

Plasmid

#146415

Purpose

Mammalian Expression of HsDCP1a-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut1 (DCP1A Human)

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniT:myc-GluA2 TKIT donor

Plasmid

#169423

Purpose

Donor DNA for TKIT knockin of Myc to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-H2B2-Venus

Plasmid

#129556

Purpose

pClimDC-H2B:mV nuclear marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

Histone 2B fused to venus

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-lifeact-mCherry

Plasmid

#129558

Purpose

pClimDCLifeAct:mCh actin filaments marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

lifeAct fused to mCherry

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-dt/deltaEGFP

Plasmid

#163919

Purpose

PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

Depositor

John Engelhardt

Article

Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

Insert

dtomato

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MNX1wt-IDR

Plasmid

#145248

Purpose

Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MNX1wt-IDR (MNX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LIC-Z-Y-L

Plasmid

#153546

Purpose

this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Expression

Mammalian

Mutation

with 6 Y in 3 ITAMs mutated to L

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSR357.20

Plasmid

#125108

Purpose

Expression of narL(1-154aa)-ydfI(148-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)154
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR322.2

Plasmid

#125117

Purpose

Expression of uhpA(1-118aa)-ydfI(121-213aa), narL126 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)126
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR359.4

Plasmid

#125115

Purpose

Expression of narL(1-159aa)-uhpA(142-129aa) chimera under PLtetO-1, output Promoter PuhpT99

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-uhpA(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.16

Plasmid

#125083

Purpose

Expression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)140
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKD284

Plasmid

#125073

Purpose

Expression of SO_4488 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4488 (SO_4488 )
lacI (lacI S286L- Please see depositor comments below)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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