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Showing: 4561 - 4580 of 8890 results

  3. ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173202
    Purpose
    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173202-plasmid-map-sequence-id-339519

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  7. ATP1A1_G2_Dual_sgRNA

    Plasmid
    #173201
    Purpose
    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173201-plasmid-map-sequence-id-339508

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  13. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100838
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1 and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    Synapsin
    Available Since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100838-plasmid-map-sequence-id-198300

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  18. pET14b-dabAB2

    Plasmid
    #133013
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Promoter
    t7
    Available Since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133013-plasmid-map-sequence-id-262987

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Showing: 4561 - 4580 of 8890 results