We narrowed to 3,869 results for: SPL;

1. pJJBAD82

   Plasmid

   #15348

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CEN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to E mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pJJBAD83

   Plasmid

   #15349

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CHN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to H mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pJJBAD84

   Plasmid

   #15350

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CIN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to I mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pJJBAD85

   Plasmid

   #15351

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CLN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to L mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pJJBAD86

   Plasmid

   #15352

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CCN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to C mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pJJBAD87

   Plasmid

   #15353

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CSN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to S mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pJJBAD88

   Plasmid

   #15354

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   DnaE C-intein(CGN)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   F to G mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET_sfCherry(1-10)_32aalinker_sfCherry(11)

   Plasmid

   #83030

   Purpose

   Bacterial expression vector for split sfCherry screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry(1-10)_32aalinker_sfCherry(11)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-30-His6-SUMO-AEY-CfaN

   Plasmid

   #122949

   Purpose

   Bacterial expression of N-intein

   Depositor

   Thomas Muir

   Article

   Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

   Insert

   CfaN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSABAD219

    Plasmid

    #45596

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    NpuDnaEC102 intein
    

    Use

    Tags

    GB1-H6

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pN1-CMV-mCherryN-IntN-DrBphP (N23)

    Plasmid

    #210724

    Purpose

    NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

    Depositor

    Vladislav Verkhusha

    Article

    Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

    Insert

    N23
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX853

    Plasmid

    #62885

    Purpose

    N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-573)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-T7-Top-T

    Plasmid

    #87309

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Top" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-T7-Bottom-T

    Plasmid

    #87310

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Bottom" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRegev_S5

    Plasmid

    #204174

    Purpose

    Single reporter with secondary structure from splicing library.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    Exon S5 in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRegev_S1

    Plasmid

    #204150

    Purpose

    Single reporter with secondary structure from splicing library.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    S1 exon in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRegev_S4

    Plasmid

    #204170

    Purpose

    Single reporter with secondary structure from splicing library.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    Exon S4 in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRegev_S2

    Plasmid

    #204162

    Purpose

    Single reporter with secondary structure from splicing library.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    S2 exon in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pComb3XSS

    Plasmid

    #63890

    Purpose

    3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    6x His, HA tag, and geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge