We narrowed to 1,028 results for: hug;

1. pAAV-EF1α-DIO-SN

   Plasmid

   #181741

   Purpose

   AAV vector for expressing SN

   Depositor

   Yasunori Hayashi

   Article

   Goto et al Science. 2021 Nov 12;374(6569):857-863.

   Insert

   SuperNova (monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPR-sgUFM1

   Plasmid

   #86133

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting UFM1

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   sgRNA targeting UFM1 (UFM1 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-sapphireAKAR-NLS

   Plasmid

   #118467

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR-NLS
   

   Use

   Tags

   Nuclear localization signal (NLS)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyn-sapphireAKAR

   Plasmid

   #118466

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-sapphireAKAR
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-sapphireAKAR(T/A)

   Plasmid

   #118465

   Purpose

   Negative-control mutant for sapphireAKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireAKAR(T/A)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRVdG-4RFP

   Plasmid

   #52489

   Purpose

   Expresses TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

   Insert

   TagRFP-T
   

   Use

   Rabies virus

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-ExRai-AKAR-Kras

   Plasmid

   #118408

   Purpose

   Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-Kras
   

   Use

   Tags

   C-terminal targeting sequence from Kras

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR-sgUFSP2

   Plasmid

   #86134

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   sgRNA targeting UFSP2 (UFSP2 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJet-CMV-CD5Flag2-bglpA

    Plasmid

    #89705

    Purpose

    Expression plasmid for Flag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD5Flag2-bglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-RAB-AKARev(T/A)

    Plasmid

    #118479

    Purpose

    Negative-control mutant for RAB-AKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-blueCKAR(T/A)

    Plasmid

    #118473

    Purpose

    Negative-control mutant for blueCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF5/FRT-DEST EVC2-TEV-YFP-Flag

    Plasmid

    #41004

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. optoPKAW196R

    Plasmid

    #104548

    Purpose

    Expresses Cry2-mCherry-PKA W196R in mammalian cells

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R

    Promoter

    CMV

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiCRISPR-v1-sgMPC2_7

    Plasmid

    #163457

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 7 targeting GPT2 (MPC2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiCRISPR-v1-sgMPC2_9

    Plasmid

    #163458

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 9 targeting GPT2 (MPC2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTL-Bag3-gRNA3

    Plasmid

    #196254

    Purpose

    Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTL-Bag2-gRNA2

    Plasmid

    #196252

    Purpose

    Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTL-Bag1-gRNA1

    Plasmid

    #196251

    Purpose

    Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTL-Bag4-gRNA4

    Plasmid

    #196255

    Purpose

    Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge