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Showing: 441 - 460 of 9103 results

  2. Flag-Jun4A-Myc

    Plasmid
    #47444
    Purpose
    mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)
    Depositor
    Axel Behrens
    Article
    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi
    Insert
    Jun 4A mutant (Jun Mouse)
    Use
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    four N-terminal residues phosphorylated by JNK, s…
    Promoter
    CMV
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    47444-plasmid-map-sequence-id-244398

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  5. pAAV-DYRK1A(K188R)-2A-mCherry

    Plasmid
    #177166
    Purpose
    Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Changed Lysine 188 to Arginine
    Promoter
    CMV with beta globin intron
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177166-plasmid-map-sequence-id-349444

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  6. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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  7. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Ryan Mehl, Richard Cooley
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  8. mCherry-CRY2-INPP5E

    Plasmid
    #79561
    Purpose
    expression of mCherry-tagged CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motif
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    INPP5E (INPP5E Human)
    Use
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    C-terminal region, aa 214_644, C641A mutation to …
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid
    #186427
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Rb7
    eGFP
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186427-plasmid-map-sequence-id-369354

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Showing: 441 - 460 of 9103 results