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pcDNA3-FLAG_FBXL7 (R310H)

Plasmid

#171849

Purpose

pcDNA3-FLAG_FBXL7 (R310H)

Depositor

Michele Pagano

Publication

Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

Insert

F-box and leucine rich repeat protein 7 (FBXL7 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Arginine 310 to Histidine

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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SHIP2-ΔPR1

Plasmid

#214902

Purpose

expression of the truncated variants of the SHIP2 without first proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 delta aa 123-410 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 123-410aa

Promoter

CMV

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-C2

Plasmid

#214906

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1189 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739, 1190-1258aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-N2

Plasmid

#214904

Purpose

expression of the truncated variants of the SHIP2 that retains first proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 123-418 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-122, 419-1258aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Publication

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Publication

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
SHIP2-C1

Plasmid

#214905

Purpose

expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 740-1258 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 1-739aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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SHIP2-N1

Plasmid

#214903

Purpose

expression of the truncated variants of the SHIP2 that retains only SH2 domain and first proline-rich domain

Depositor

Pavel Krejčí

Publication

Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

Insert

human SHIP2 aa 1-418 (INPPL1 Human)

Tags

V5/His

Expression

Mammalian

Mutation

delta 419-1258aa

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNTPTP alpha (Y789F)

Plasmid

#17702

Depositor

Xinmin Zheng

Publication

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha Y789F (PTPRA Human)

Expression

Mammalian

Mutation

Y789F

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

Plasmid

#210508

Purpose

expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells

Depositor

Dongmin Lee

Publication

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

Tags

GB1

Expression

Mammalian

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Fgr-SH2-AviTag

Plasmid

#214205

Purpose

Bacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Publication

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Fgr kinase SH2 domain (FGR Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTPTP alpha (S204A)

Plasmid

#17694

Depositor

Xinmin Zheng

Publication

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Tags

HA

Expression

Mammalian

Mutation

S204A

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S180A/S204A)

Plasmid

#17700

Depositor

Xinmin Zheng

Publication

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S180A S204A (PTPRA Human)

Expression

Mammalian

Mutation

S180A / S204A

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Publication

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSH1/S-Fvls-p30Casp9-E

Plasmid

#15272

Depositor

David Spencer

Publication

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36)-p30Caspase9 (CASP9 Human)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12 F36 to VTruncated Caspase9 (135-416; lacki…

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSH1/M-Fv-Casp3-E

Plasmid

#15271

Depositor

David Spencer

Publication

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36)-Caspase3 (CASP3 Human)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12(V36) F36->V

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pN1-Lck-GCaMP2

Plasmid

#24794

Depositor

Baljit Khakh

Publication

Shigetomi et al Nat Neurosci. 2010 Jun;13(6):759-66.

Insert

Lck-GCaMP2

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR1-Fv-Fvls-E

Plasmid

#15285

Depositor

David Spencer

Publication

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FGFR1 cytoplasmic domain FKBP12 F36V

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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myristoylated ct-GRK2

Plasmid

#178734

Purpose

N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

Depositor

Ege Kavalali

Publication

Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

Insert

GRK2 (GRK2 Bovine)

Use

Lentiviral

Tags

Src 1-15

Mutation

only amino acids 548-671

Promoter

Ubiquitin

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNTPTP alpha (S204A)

Plasmid

#17699

Depositor

Xinmin Zheng

Publication

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Expression

Mammalian

Mutation

S204A

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only