We narrowed to 14,304 results for: TIM;

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pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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NEK7 gRNA (BRDN0001162196)

Plasmid

#77740

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK7

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK7 (NEK7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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SspB-mCherry-DHPH-TIAM1

Plasmid

#176104

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001145952)

Plasmid

#76714

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

Plasmid

#133789

Purpose

CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized CjeCas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Mutation

n/a

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR4-EGFP_opt

Plasmid

#106334

Purpose

Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

EGFP

Use

Gateway entry vector

Expression

Bacterial and Mammalian

Mutation

codon optimized for gene synthesis without affect…

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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TK1 gRNA (BRDN0001145069)

Plasmid

#76424

Purpose

3rd generation lentiviral gRNA plasmid targeting human TK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TK1 (TK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001162598)

Plasmid

#75577

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001146004)

Plasmid

#80174

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shJUND puro

Plasmid

#58698

Purpose

Lentiviral shRNA vector for knockdown of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shJUND (JUND Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pET28a(ICOS)

Plasmid

#177903

Purpose

Bacterial expression of his10-tagged human ICOS Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

ICOS (aa 162-199 only) (ICOS Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-mKate2

Plasmid

#199249

Purpose

Fluorescent labelling of bacterial cells with mKate2

Depositor

Uwe Mamat

Article

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized mKate2 gene

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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PKN3 gRNA (BRDN0001146586)

Plasmid

#77947

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149423)

Plasmid

#80255

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001147733)

Plasmid

#75576

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NUAK1 gRNA (BRDN0001162525)

Plasmid

#75578

Purpose

3rd generation lentiviral gRNA plasmid targeting human NUAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NUAK1 (NUAK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-CD80-mGFP

Plasmid

#223591

Purpose

Lentiviral expression of human CD80 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PHKG1 gRNA (BRDN0001144981)

Plasmid

#76356

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKG1 (PHKG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PFKFB4 gRNA (BRDN0001149495)

Plasmid

#77123

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB4 (PFKFB4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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STK17A gRNA (BRDN0001149480)

Plasmid

#77916

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK17A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK17A (STK17A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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