Skip to main content
Addgene
Showing: 4581 - 4600 of 6828 results

  1. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

    Enlarge

  4. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169389-plasmid-map-sequence-id-336165

    Enlarge

  5. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

    Enlarge

  6. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

    Enlarge

  8. pmCellFree_KA1_SARS-COV-2_M

    Plasmid
    #169400
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus M with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _M (M SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169400-plasmid-map-sequence-id-336863

    Enlarge

  9. pmCellFree_KA1_SARS-COV-2_E

    Plasmid
    #169399
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus E with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _E (E SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169399-plasmid-map-sequence-id-335422

    Enlarge

  10. pmCellFree_KA1_SARS-COV-2_N

    Plasmid
    #169401
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _N (N SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169401-plasmid-map-sequence-id-336866

    Enlarge

  11. pmCellFree_KA1_SARS-COV-2_S

    Plasmid
    #169402
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus S with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _S (S SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169402-plasmid-map-sequence-id-336167

    Enlarge

  12. pmCellFree_KA1_SARS-COV-2_ORF10

    Plasmid
    #169398
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF10 (ORF10 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169398-plasmid-map-sequence-id-336872

    Enlarge

  13. pmCellFree_KA1_SARS-COV-2_ORF8

    Plasmid
    #169395
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF8 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF8 (ORF8 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    169395-plasmid-map-sequence-id-336176

    Enlarge

  14. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid
    #169376
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP1
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169376-plasmid-map-sequence-id-335277

    Enlarge

  15. pmCellFree_KA1_SARS-COV-2_ORF6

    Plasmid
    #169392
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169392-plasmid-map-sequence-id-335430

    Enlarge

  16. pNK-TGCK

    Plasmid
    #45359
    Depositor
    Sean Wu
    Inserts
    tTA
    eGFP-Cre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Nkx2.5 cardiac enhancer and promoter fragme…
    Available since
    Aug. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    45359-plasmid-map-sequence-id-393051

    Enlarge

  17. pEG608

    Plasmid
    #40194
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…
    Expression
    Worm
    Mutation
    Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only

  18. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

    Plasmid
    #183607
    Purpose
    Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    LaG17 GFP nanobody SynNotch tTA
    Use
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Pgk1
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183607-plasmid-map-sequence-id-361593

    Enlarge

  19. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid
    #169390
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169390-plasmid-map-sequence-id-336867

    Enlarge

  20. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid
    #169394
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169394-plasmid-map-sequence-id-336882

    Enlarge

Showing: 4581 - 4600 of 6828 results