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pOPINO_H11-B5

Plasmid

#184279

Purpose

Expresses nanobody H11-B5 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-B5

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINO_11-H6

Plasmid

#184280

Purpose

Expresses nanobody H11-H6 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H6

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINH11-H4 Q98R_H100E

Plasmid

#184281

Purpose

Expresses nanobody H11-H4 Q98R_H100E in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11-H4 Q98R_H100E

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.1 R57A, R59A pMT2

Plasmid

#206112

Purpose

expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

Depositor

Annette Dolphin

Article

Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Mutation

R57A, R59A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CRF0.5

Plasmid

#208657

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199, 330, 332N IRES GFP

Plasmid

#195703

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199, 330, 332N …

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 HA D491A pcDNA3

Plasmid

#206070

Purpose

expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

Depositor

Annette Dolphin

Article

Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

D491A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA9

Plasmid

#196108

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146490

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H

Plasmid

#146491

Purpose

Insect Expression of DmPABPC1-RRM2-RRM3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM2-RRM3 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H

Plasmid

#146492

Purpose

Insect Expression of DmPABPC1-RRM3-RRM4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM3-RRM4 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146479

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCav1 in pT3TS-Dest

Plasmid

#194293

Purpose

In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin (cav1.S Frog)

Use

In vitro transcription of mrna

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

Plasmid

#192233

Purpose

lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192234

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192232

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RPS4X sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192231

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1a

Plasmid

#194291

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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mTln1-F2F3-P229L-pET151

Plasmid

#177879

Purpose

Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

changed Proline 229 to Leucine (P229L)

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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