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pHH0103_NOXO1-1/2

Plasmid

#91499

Purpose

Protein expression and purification of human SH3 domain construct NOXO1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOXO1-1/2 (NOXO1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR/U6 HDAC4 shRNA

Plasmid

#32220

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against murine HDAC4 (Hdac4 Mouse)

Use

RNAi; Gateway u6 entry vector

Promoter

human U6

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_SHANK3-1/1

Plasmid

#91421

Purpose

Protein expression and purification of human SH3 domain construct SHANK3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK3-1/1 (SHANK3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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mWasabi-Mito-7

Plasmid

#56508

Purpose

Localization: Mitochondria, Excitation: 493, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mWasabi

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pENTR/U6 HDAC5 shRNA

Plasmid

#32222

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against mouse HDAC5 (Hdac5 Mouse)

Use

RNAi; Gateway u6 entry vector

Promoter

human U6

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
TTR C10S S85C K80D

Plasmid

#184743

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, K80D

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFA0055

Plasmid

#131774

Purpose

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer CASS5a guide (gCASS5a) and 5' sgRNA

Expression

Bacterial and Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GL3-1/1

Plasmid

#91423

Purpose

Protein expression and purification of human SH3 domain construct SH3GL3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GL3-1/1 (SH3GL3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPKm-196

Plasmid

#90511

Purpose

pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PIF6-DBD

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-mCitrine

Plasmid

#61676

Purpose

Single mCitrine-labeled, truncated kinesin-1

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KHC(1-560)-mCitrine (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK1-1/1-SV1/3

Plasmid

#91548

Purpose

Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK1-1/1-SV1/3 (TNK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-GFP11

Plasmid

#61670

Purpose

Non-fluorescent, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAd FLAG HDAC5 S259A/S498A

Plasmid

#32208

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

S259A/S498A

Available Since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_CEP41_HaloTag9

Plasmid

#169331

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159870

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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p1.2-Zeo-eGFP

Plasmid

#162743

Purpose

Fluorescent reporter for CHO cells expression studies

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Insert

enhanced green fluorescent protein

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-PH-citrine

Plasmid

#131406

Purpose

Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine

Depositor

Lars Hufnagel

Article

Guignard et al Science. 2020 Jul 10;369(6500). pii: 369

Insert

PH

Use

In vitro transcription

Tags

citrine yellow fluorescent protein

Promoter

T7

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGMC00013

Plasmid

#172525

Purpose

sgRNA against mouse Mr1

Depositor

Raj Chari

Article

Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

Insert

Mr1 (Mr1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159867

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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