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pPRIME-CMV-GFP-shCrh2

Plasmid

#132710

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(2) (Crh Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154A/H250Y

Plasmid

#108491

Purpose

expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP

Plasmid

#108500

Purpose

inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP d543-746iPRC1 304-509 (PRC1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

delta alpha Helix, insert PRC1 microtubule domain

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-vsv-Incenp d543-746iPRC1_279-482-GFP

Plasmid

#108504

Purpose

inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP d543-746iPRC1 304-509 (PRC1 Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

delta alpha Helix, insert PRC1 microtubule domain

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-INCENP d543-746

Plasmid

#108473

Purpose

expression of vsv-INCENP d543-746

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

INCENP d543-746 AA (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

Delta Alpha helix, aa543-746 deleted

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-AuroraB L154A/H250Y

Plasmid

#108489

Purpose

expression of vsv-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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E2AK2_HUMAN_D0

Plasmid

#79712

Purpose

This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

E2AK2 (EIF2AK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His

Plasmid

#83458

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

Plasmid

#61796

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

Plasmid

#61793

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

Plasmid

#61791

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

Plasmid

#61790

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

Plasmid

#61786

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
CFP(1-158)-RGS7t

Plasmid

#55761

Purpose

An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

RGS7(202-477)-CFP(1-158) (RGS7 Aequorea victoria, Human)

Tags

CFP(1-158) was fused to the amino terminus of RGS…

Expression

Mammalian

Mutation

The RGS sequence amino terminal to the GGL domain…

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

Plasmid

#61787

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR with miR-200c MRE-2 mutated

Use

Luciferase

Mutation

miR-200c MRE-2 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

Plasmid

#61788

Purpose

Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Tob1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

Plasmid

#61794

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

Plasmid

#61795

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only