We narrowed to 5,339 results for: pcdna3.1

1. hHSF1 K298R

   Plasmid

   #118362

   Purpose

   This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K298R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FL-BCL2L1

   Plasmid

   #78860

   Purpose

   DULIP positive control (prey) for assay establishment

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   BCL2 like 1 (BCL2L1 Human)
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. LUC2 (L/Sec/deltaP)

   Plasmid

   #112145

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and deleted AUGA in PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
   

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and d…

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. VBS mutant of alpha catenin sensor

   Plasmid

   #71710

   Purpose

   This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin ΔVBS FRET Sensor
   

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Deleted amino acid residues 316-405 in α -catenin…

   Promoter

   CMV

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSP14-Flag

   Plasmid

   #210346

   Purpose

   Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Opto-GPR151

   Plasmid

   #106051

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR151; Opto-GPR151; E5)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR151 (GPR151 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-MED27

   Plasmid

   #15424

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED27 (MED27 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. LRRK2-K1906M

   Plasmid

   #35946

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pGlomyc-Klf4

   Plasmid

   #172870

   Purpose

   Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

   Depositor

   Renee van Amerongen

   Article

   Mourao et al bioRxiv 2021.06.22.449461

   Insert

   Klf4 (Klf4 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LUC45 (L/Sec/mutP)

    Plasmid

    #112148

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258 mutated to selenocysteine (U) and SE…

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-MED21

    Plasmid

    #15422

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED21 (MED21 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pTE4495

    Plasmid

    #80338

    Purpose

    mammalian expression MbCpf1 nuclease and Mb crRNA

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    MbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Promoter

    CMV and human U6

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-bJun-C-mSOG-IRES-mCherry

    Plasmid

    #136600

    Purpose

    Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    bJun-C-mSOG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Opto-GPR18

    Plasmid

    #106006

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR18; Opto-GPR18; A8)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR18 (GPR18 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. F-MED28

    Plasmid

    #15368

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED28 (MED28 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-sACE2-WT(732)

    Plasmid

    #154098

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Elafin

    Plasmid

    #18102

    Depositor

    Ronny Drapkin

    Article

    Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

    Insert

    Elafin (PI3 Human)
    

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Opto-GPR12

    Plasmid

    #106003

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR12; Opto-GPR12; A5)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR12 (GPR12 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-Cx43-YA/VD-GFP

    Plasmid

    #60703

    Purpose

    mammalian expression of rat Cx43 YA/VD mutant fused to GFP

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    YA/VD (Y286A/V289D)

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only