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pDASH-DI-α2

Plasmid

#242516

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, PPP2R1A-HaloTag

Plasmid

#238714

Purpose

Express PPP2R1A-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

PPP2R1A (PPP2R1A Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDBB31

Plasmid

#248962

Purpose

DD-GAVPO (degron-regulated GAVPO) expression under control of the EF1alpha promoter in a PiggyBac integration plasmid

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Insert

DD-GAVPO

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pQF-lacZ

Plasmid

#48094

Purpose

Cumate-inducible expression of lacZ

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Insert

E. coli lacZ

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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MyD116.PFLAG.CMV2

Plasmid

#21834

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 (Ppp1r15a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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haA1.pBABEpu

Plasmid

#21813

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 C-term

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2

Plasmid

#242520

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQR

Plasmid

#48099

Purpose

Cumate-inducible expression of mCherry-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and mCherry

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6-11 1397C

Plasmid

#221933

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MyD116.delC.pBABEpu

Plasmid

#21835

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

GADD34 (Ppp1r15a Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pRS2 (HIF2a-pRetro-Super)

Plasmid

#22100

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRS9 (HIF2a-pRetro-Super)

Plasmid

#22101

Depositor

William Kaelin

Article

Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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T_antiEGFPsynsrRNA_UA

Plasmid

#185974

Purpose

Expresses antiEGFPsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

PlTetO-1 AntiEGFPsynsrRNA

Use

Synthetic Biology

Promoter

PlTetO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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L_EGFP_AC

Plasmid

#185971

Purpose

Expresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

Pl-LacO1-Egfp

Use

Synthetic Biology

Promoter

PlLacO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pQHD

Plasmid

#48102

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of HA-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

HA tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQFD

Plasmid

#48101

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pQYD

Plasmid

#48104

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of SYFP2-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

SYFP2

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMH4-SYN-ChR2-tdimer2RFP

Plasmid

#22772

Depositor

Thomas Oertner

Article

Zhang et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

ChR2-tdimer2RFP

Tags

ChR2-tdimer2RFP

Expression

Mammalian

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pTLNXA7

Plasmid

#49033

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

chicken alpha7 signal sequence

Promoter

SP6

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDBB57

Plasmid

#248964

Purpose

Scube2 expression under control of the EF1alpha promoter in a PiggyBac integration plasmid

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Insert

Scube2

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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