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pDONR223-PRKAB1

Plasmid

#23360

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAB1 (PRKAB1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-CAMK2D

Plasmid

#23814

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK2D (CAMK2D Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcDNA3-YFP-PKD2

Plasmid

#31821

Depositor

Alexandra Newton

Publication

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YFP-Protein Kinase D2 (PRKD2 Human)

Tags

YFP

Expression

Mammalian

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pBE14

Plasmid

#174653

Purpose

In vivo Cre-lox circularization in E. coli

Depositor

Huimin Zhao

Publication

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

Cre

Use

Synthetic Biology

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMyNT-kan

Plasmid

#84693

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Publication

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pattB-QF-sv40

Plasmid

#24367

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ABE8.8-m

Plasmid

#136294

Purpose

expresses ABE8.8-m in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.8-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Publication

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDK8 (CDK8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pETM6-E12-vioABECD

Plasmid

#66537

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC8 human IFN-gamma

Plasmid

#17600

Depositor

Howard Young

Publication

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

interferon-gamma cDNA (IFNG Human)

Expression

Bacterial

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Publication

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Publication

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNCA (SNCA Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Publication

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Publication

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PKC epsilon DN

Plasmid

#21243

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC epsilon (Prkce Mouse)

Tags

HA

Expression

Mammalian

Mutation

K437R

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET Flag TEV LIC cloning vector (1L)

Plasmid

#29662

Depositor

Scott Gradia

Type

Empty backbone

Tags

Flag and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR1-Fv-Fvls-E

Plasmid

#15285

Depositor

David Spencer

Publication

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FGFR1 kinase, FKBP12v36 (Fgfr1 Mouse)

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FGFR1 cytoplasmic domain FKBP12 F36V

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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6xHis-SNAP-PI(3,4)P2 Probe

Plasmid

#211511

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

TAPP1 cPHx3

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSUMO3

Plasmid

#52260

Purpose

Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Myr.PKCzeta.FLAG

Plasmid

#10802

Depositor

Alex Toker

Publication

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG and Myr

Expression

Mammalian

Available since

July 26, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles