We narrowed to 25,582 results for: lenti

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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dCas9-hHDAC3

Plasmid

#98591

Purpose

3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCAS9-HDAC3 (HDAC3 S. pyogenes, Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre stuffer v4

Plasmid

#158032

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Cas9-sgRNA-A+B

Plasmid

#149370

Purpose

Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
sgRNA-B
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CBh, PGK, and U6

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_062

Plasmid

#123951

Purpose

Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR DEST

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK467] pLJC5 HA-Depdc5

Plasmid

#109343

Purpose

Lenti-viral expression of HA-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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[pSK468] pLJC5 Flag-Depdc5

Plasmid

#109337

Purpose

Lenti-viral expression of Flag-tagged wildtype Depdc5

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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PLX306 iCre tetO NICD rtTA

Plasmid

#158049

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of mouse Notch intracellular domain

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

mouse Notch Intra Cellular Domain

Tags

V5

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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H446 EF1a RA-ABE6.3 p2A Puro

Plasmid

#139104

Purpose

Lentiviral expression of ABE6.3.

Depositor

Wen Xue

Article

Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

Insert

ABE RA6.3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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[pSK475] pLJC5 HA-Depdc5(A)

Plasmid

#109344

Purpose

Lenti-viral expression of HA-tagged Depdc5 mutant A

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

HA

Mutation

DIYGD(185-189)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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JPF0454

Plasmid

#124039

Purpose

Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-spCAS9-P2A-mAzamiGreen

Expression

Mammalian

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgUroD v4

Plasmid

#158036

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

UroD sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgItgb5 v3

Plasmid

#158038

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Itgb5 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre sgRipk4 v3

Plasmid

#158035

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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JPF0464

Plasmid

#124035

Purpose

Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pEF1a-iRFP713_non targeting sgRNA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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