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pUC19_PHF1_KO_BSD_donor

Plasmid

#246396

Purpose

PHF1 KO donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_HTC3xF_BSD_donor

Plasmid

#246398

Purpose

PHF19 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_KO_BSD_donor

Plasmid

#246400

Purpose

PHF19 KO donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p-TID-FKBP-HA3

Plasmid

#228006

Purpose

Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

HIS3

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-3HA-KanMX6

Plasmid

#228024

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQAT10

Plasmid

#223382

Purpose

T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpRY-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pAG1345 mCherry-FIREtag-DEVDG-MoA

Plasmid

#205176

Purpose

Expresses mCherry-FIREtag-DEVDG-MoA in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-cagedFIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pOGG202

Plasmid

#115503

Purpose

Clone of a level 1 unit which encodes IPTG-inducible sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tipin

Plasmid

#22889

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
TLCV2 - sgPTEN

Plasmid

#202759

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting PTEN

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Promoter

CMV-SP6

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9_CL

Plasmid

#166245

Purpose

regulated dCas9 generator

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

P112-sgRNA-term-P104-RBS1-dCas9-B15

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ec-TTL-P112 and Ec-TTL-P104

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pmNFL

Plasmid

#83127

Purpose

Mouse neurofilament protein L cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

mouse NFL (Nefl Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-3HaloTag

Plasmid

#179828

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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RCASBP-AP (A) (CC#37)

Plasmid

#15160

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pBHM2675

Plasmid

#241722

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEXP(V5-APEX2-NES)

Plasmid

#107596

Purpose

Expresses V5-APEX2-NES in mammalian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

APEX2-NES

Use

Frt site to insert into hek293 t-rex genome

Tags

NES and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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