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C098

Plasmid

#174345

Purpose

Template for making Nluc mRNA reporter with pA139.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Available Since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

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C079

Plasmid

#174348

Purpose

Template for making mRNA for overexpressing eGFP in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eGFP

Use

In vitro transcription template

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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C035

Plasmid

#174341

Purpose

Template for making Rluc mRNA reporter with pA120.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAM111

Plasmid

#86481

Purpose

Expresses p9-6His-tnaA

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p9-6His-tnaA

Use

Synthetic Biology

Tags

6His and tnaA

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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BioBrick pET-24a(+)

Plasmid

#117802

Purpose

BioBrick pET vector for protein overexpression in E. coli

Depositor

Anthony Forster

Article

Du et al Biotechnol Bioeng. 2009 Dec 15;104(6):11

Type

Empty backbone

Use

Escherichia coli

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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IMBB2.4-pUC57-mini

Plasmid

#88845

Purpose

plasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2017 Mar 1;62(3):99-106.

Type

Empty backbone

Use

Synthetic Biology; Cloning

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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C034

Plasmid

#174340

Purpose

Template for making Rluc mRNA reporter with pA98.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C033

Plasmid

#174339

Purpose

Template for making Rluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C031

Plasmid

#174338

Purpose

Template for making Rluc mRNA reporter with pA30.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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p307HU

Plasmid

#27386

Depositor

David Bartel

Article

Shechner et al Science. 2009 Nov 27. 326(5957):1271-5.

Insert

unligated form of ligase ribozyme

Expression

Bacterial

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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C255

Plasmid

#174352

Purpose

Template for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

eGFP

Use

In vitro transcription template

Tags

FLAG and HA

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAM12

Plasmid

#86471

Purpose

Expresses p12 and p9-mCitrine

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9-mCitrine

Use

Synthetic Biology

Tags

mCitrine

Expression

Bacterial

Promoter

pBAD

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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C071

Plasmid

#174343

Purpose

Template for making Nluc mRNA reporter with pA29.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Available Since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C093

Plasmid

#174344

Purpose

Template for making Nluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Available Since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBR322 14-3-3 Promoter

Plasmid

#16517

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

14-3-3 promoter (SFN Human)

Expression

Bacterial

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pGwf

Plasmid

#22517

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCDEF3-muSOX

Plasmid

#131695

Purpose

expresses muSOX in mammalian cells

Depositor

Britt Glaunsinger

Article

Hartenian et al bioRxiv 585984

Insert

muSOX (GAMMAHV.ORF37 MHV68)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQE-WT-T3-R5-RV

Plasmid

#63628

Purpose

T3-R5-RV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3-R5-RV RNAP

Mutation

T745K, N748D, L749M, M750I, H772R, E775V

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCAM35

Plasmid

#86475

Purpose

Expresses p9-6His

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p9-6His

Use

Synthetic Biology

Tags

6His

Expression

Bacterial

Promoter

pTet

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS (A) shh (CT#251)

Plasmid

#13991

Depositor

Cliff Tabin

Article

Riddle et al Cell. 1993 Dec 31. 75(7):1401-16.

Insert

shh (SHH Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only