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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,810 results for: itch

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Showing: 4661 - 4680 of 7810 results

  3. pCDNA3-LOV-VP16

    Plasmid
    #42499
    Purpose
    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor
    Depositor
    Ricardo Dolmetsch
    Article
    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d
    Insert
    FKF1 (N-LOV, G128D mutant) fused with VP16
    Tags
    F-type VP16 activation domain and HA tag
    Expression
    Mammalian
    Mutation
    G128D 1-168aa
    Promoter
    CMV
    Available Since
    March 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    42499-plasmid-map-sequence-id-57336

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  4. pcDNA3-N-I-N-CAAX

    Plasmid
    #41686
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Available Since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41686-plasmid-map-sequence-id-55893

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  6. pcDNA3-K-I-N-CAAX

    Plasmid
    #41640
    Purpose
    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    Tags
    CAAX box
    Expression
    Mammalian
    Mutation
    K145N on Dronpa
    Promoter
    CMV
    Available Since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41640-plasmid-map-sequence-id-240702

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  10. pRMH120

    Plasmid
    #40593
    Purpose
    Expresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expression
    Depositor
    Chandra Tucker
    Article
    Hughes et al Methods. 2012 Aug 15.
    Inserts
    CRY2 (CRY2 Mustard Weed)
    CIB1 (CIB1 Mustard Weed)
    Expression
    Yeast
    Promoter
    ADH1 and TEF
    Available Since
    Oct. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    40593-plasmid-map-sequence-id-52556

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  15. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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  16. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid
    #237479
    Purpose
    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    M210A + F213A
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    237479-plasmid-map-sequence-id-476565

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Showing: 4661 - 4680 of 7810 results