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pdest myr-AKT2 puro

Plasmid

#223053

Purpose

myr-AKT2 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

myr-AKT2 (Akt2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro

Plasmid

#195567

Purpose

PiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cells

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

TIR1

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYX-N-Puro-mNeon-TSC22D1

Plasmid

#222667

Purpose

endogenously tag TSC22D1 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D1 (TSC22D1 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X S404N

Plasmid

#225724

Purpose

Transfection of USP27X (S404N) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X S404N (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

S404N

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X F313V

Plasmid

#225722

Purpose

Transfection of USP27X (F313V) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X F313V (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

F313V

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X K181E

Plasmid

#225721

Purpose

Transfection of USP27X (K181E) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X K181E (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

K181E

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X Y144C

Plasmid

#225720

Purpose

Transfection of USP27X (Y144C) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X Y144C (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

Y144C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro HA-hUSP27X G76S

Plasmid

#225719

Purpose

Transfection of USP27X (G76S) with HA tag in mammalian cells

Depositor

Francisco Bustos

Article

Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

Insert

USP27X G76S (USP27X Human)

Tags

HA

Expression

Mammalian

Mutation

G76S

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gATRIP_A

Plasmid

#211537

Purpose

sgRNA-A against ATRIP

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ATRIP

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-BCL9

Plasmid

#202662

Purpose

Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_short_mCherry2-NLS-PEST_Puro

Plasmid

#213774

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT4_mCherry2-NLS-PEST_Puro

Plasmid

#213773

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT4_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT3_short_mCherry2-NLS-PEST_Puro

Plasmid

#213772

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT3_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_mCherry2-NLS-PEST_Puro

Plasmid

#213769

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT2_short_mCherry2-NLS-PEST_Puro

Plasmid

#213770

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT2_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_mCherry2-NLS-PEST_Puro

Plasmid

#213767

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-HKGT1_short_mCherry2-NLS-PEST_Puro

Plasmid

#213768

Purpose

Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

HKGT1_short_mCherry2-NLS-PEST

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LV-sgRNA-mCherry-Puro sgPIDD

Plasmid

#211528

Purpose

Deletes PIDD

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgPIDD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gRAD9A_B

Plasmid

#211612

Purpose

sgRNA-B against RAD9

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA RAD9A

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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