We narrowed to 6,200 results for: Pet2;

1. pF711-pET28a-Hs-delta25-MCAD-NHis wt

   Plasmid

   #85112

   Purpose

   expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta25) MCAD (ACADM Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21b-LOV-ipaA L514K L531E

   Plasmid

   #40253

   Depositor

   Brian Kuhlman

   Article

   Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

   Insert

   LOV-ipaA L514K L531E
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L514K; L531E; Jα helix region hybridized (AsLOV2 …

   Promoter

   T7

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-ybbr-HIS-ddFLN4-Fgß

   Plasmid

   #101719

   Purpose

   wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
   

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPV00605 pET21GG2-His(6)-BECN(1-265)

   Plasmid

   #136686

   Purpose

   Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 IDR + CC (BECN1 Human)
   

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FL MALT1 Catalytic Mutant in pET29b-Strep

   Plasmid

   #48969

   Purpose

   Expresses Full length catalytic mutant MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Catalytic Mutant C464A

   Promoter

   T7

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a SUMO Nup53xl 162-267

   Plasmid

   #86002

   Purpose

   bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Type

   Empty backbone

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-FgßF3-ddFLN4-HIS-ybbr

   Plasmid

   #101743

   Purpose

   mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
   

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4, added 9F in FgßF3

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. delNC C-His Ana GvpC in pET28a

    Plasmid

    #85736

    Purpose

    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (truncated and his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Deletion of N and C terminal regions, addition of…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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